Universidade de Pernambuco

About: Universidade de Pernambuco is a education organization based out in Recife, Brazil. It is known for research contribution in the topics: Population & Medicine. The organization has 6147 authors who have published 6948 publications receiving 73648 citations.

Geographic distribution of hepatitis C virus genotypes in Brazil

Abstract: Brazil is a country of continental dimension with a population of different ethnic backgrounds. Thus, a wide variation in the frequencies of hepatitis C virus (HCV) genotypes is expected to occur. To address this point, 1,688 sequential samples from chronic HCV patients were analyzed. HCV-RNA was amplified by the RT-PCR from blood samples collected from 1995 to 2000 at different laboratories located in different cities from all Brazilian States. Samples were collected in tubes containing a gel separator, centrifuged in the site of collection and sent by express mail in a refrigerated container to Laboratorio Bioquimico Jardim Paulista, Sao Paulo, SP, Brazil. HCVRNA was extracted from serum and submitted to RT and nested PCR using standard procedures. Nested PCR products were submitted to cycle sequencing reactions without prior purification. Sequences were analyzed for genotype determination and the following frequencies were found: 64.9% (1,095) for genotype 1, 4.6% (78) for genotype 2, 30.2% (510) for genotype 3, 0.2% (3) for genotype 4, and 0.1% (2) for genotype 5. The frequencies of HCV genotypes were statistically different among Brazilian regions (P = 0.00017). In all regions, genotype 1 was the most frequent (51.7 to 74.1%), reaching the highest value in the North; genotype 2 was more prevalent in the Center-West region (11.4%), especially in Mato Grosso State (25.8%), while genotype 3 was more common in the South (43.2%). Genotypes 4 and 5 were rarely found and only in the Southeast, in Sao Paulo State. The present data indicate the need for careful epidemiological surveys throughout Brazil since knowing the frequency and distribution of the genotypes would provide key information for understanding the spread of HCV.

II Consenso Brasileiro em Doença de Chagas, 2015

Abstract: RESUMO A doenca de Chagas e uma condicao cronica negligenciada com elevada carga de morbimortalidade e impacto dos pontos de vista psicologico, social e economico. Representa um importante problema de saude publica no Brasil, com diferentes cenarios regionais. Este documento traduz a sistematizacao das evidencias que compoe o Consenso Brasileiro de Doenca de Chagas. O objetivo foi sistematizar estrategias de diagnostico, tratamento, prevencao e controle da doenca de Chagas no pais, de modo a refletir as evidencias cientificas disponiveis. Sua construcao fundamentou-se na articulacao e contribuicao estrategica de especialistas brasileiros com conhecimento, experiencia e atualizacao sobre diferentes aspectos da doenca. Representa o resultado da estreita colaboracao entre a Sociedade Brasileira de Medicina Tropical e o Ministerio da Saude. Espera-se com este documento fortalecer o desenvolvimento de acoes integradas para enfrentamento da doenca no pais com foco em epidemiologia, gestao, atencao integral (incluindo familias e comunidades), comunicacao, informacao, educacao e pesquisas.

Diagnosis and treatment of dentinal hypersensitivity

Abstract: This bibliographic review provides a general view of the etiology, characteristics and treatment of dentinal hypersensitivity, so that professionals can use this information in the therapeutic management of this clinical condition. For this purpose, the authors have analyzed whole texts of relevant articles on the subject. This study showed that the predisposing factors associated with the causes of dentinal hypersensitivity must be controlled or eliminated, by educating the patient regarding the excessive intake of acidic food, as well as providing guidance on the proper tooth brushing technique and analysis of occlusion. Effective treatment must be preceded by a proper diagnosis, established after the exclusion of any other possible causes of the pain. These cases must be managed efficiently, quickly and permanently. The availability of a wide variety of treatment could be an indicator that there is still no effective desensitizing agent to completely resolve the patient's discomfort, or that it is difficult to treat, irrespective of the available treatment options. Even with the large number of published studies, it has not been possible to reach a consensus about the product that represents the gold standard in the treatment of dentinal hypersensitivity. (J Oral Sci 51, 323-332, 2009)