University of Cagliari

About: University of Cagliari is a education organization based out in Cagliari, Italy. It is known for research contribution in the topics: Population & Dopamine. The organization has 11029 authors who have published 29046 publications receiving 771023 citations. The organization is also known as: Università degli Studi di Cagliari & Universita degli Studi di Cagliari.

The 6-hydroxydopamine model of Parkinson's disease.

Abstract: The neurotoxin 6-hydroxydopamine (6-OHDA) continues to constitute a valuable topical tool used chiefly in modeling Parkinson’s disease in the rat. The classical method of intracerebral infusion of 6-OHDA, involving a massive destruction of nigrostriatal dopaminergic neurons, is largely used to investigate motor and biochemical dysfunctions in Parkinson’s disease. Subsequently, more subtle models of partial dopaminergic degeneration have been developed with the aim of revealing finer motor deficits. The present review will examine the main features of 6-OHDA models, namely the mechanisms of neurotoxin-induced neurodegeneration as well as several behavioural deficits and motor dysfunctions, including the priming model, modeled by this means. An overview of the most recent morphological and biochemical findings obtained with the 6-OHDA model will also be provided, particular attention being focused on the newly investigated intracellular mechanisms at the striatal level (e.g., A2A and NMDA receptors, PKA, CaMKII, ERK kinases, as well as immediate early genes, GAD67 and peptides). Thanks to studies performed in the 6-OHDA model, all these mechanisms have now been hypothesised to represent the site of pathological dysfunction at cellular level in Parkinson’s disease.

Early effects of gliadin on enterocyte intracellular signalling involved in intestinal barrier function

Abstract: Background and aims: Despite the progress made in understanding the immunological aspects of the pathogenesis of coeliac disease (CD), the early steps that allow gliadin to cross the intestinal barrier are still largely unknown. The aim of this study was to establish whether gliadin activates a zonulin dependent enterocyte intracellular signalling pathway(s) leading to increased intestinal permeability. Methods: The effect of gliadin on the enterocyte actin cytoskeleton was studied on rat intestinal epithelial (IEC-6) cell cultures by fluorescence microscopy and spectrofluorimetry. Zonulin concentration was measured on cell culture supernatants by enzyme linked immunosorbent assay. Transepithelial intestinal resistance (Rt) was measured on ex vivo intestinal tissues mounted in Ussing chambers. Results: Incubation of cells with gliadin led to a reversible protein kinase C (PKC) mediated actin polymerisation temporarily coincident with zonulin release. A significant reduction in Rt was observed after gliadin addition on rabbit intestinal mucosa mounted in Ussing chambers. Pretreatment with the zonulin inhibitor FZI/0 abolished the gliadin induced actin polymerisation and Rt reduction but not zonulin release. Conclusions: Gliadin induces zonulin release in intestinal epithelial cells in vitro. Activation of the zonulin pathway by PKC mediated cytoskeleton reorganisation and tight junction opening leads to a rapid increase in intestinal permeability.

Analysis of Chattering in Systems With Second-Order Sliding Modes

Abstract: A systematic approach to the chattering analysis in systems with second-order sliding modes is developed. The neglected actuator dynamics is considered to be the main cause of chattering in real systems. The magnitude of oscillations in nonlinear systems with unmodeled fast nonlinear actuators driven by second-order sliding-mode control generalized suboptimal (2-SMC G-SO) algorithms is evaluated. Sufficient conditions for the existence of orbitally stable periodic motions are found in terms of the properties of corresponding Poincare maps. For linear systems driven by 2-SMC G-SO algorithms, analysis tools based on the frequency-domain methods are developed. The first of these techniques is based on the describing function method and provides for a simple approximate approach to evaluate the frequency and the amplitude of possible periodic motions. The second technique represents a modified Tsypkin's method and provides for a relatively simple, theoretically exact, approach to evaluate the periodic motion parameters. Examples of analysis and simulation results are given throughout this paper.

On multi-input chattering-free second-order sliding mode control

Abstract: A solution to the problem of eliminating the chattering effect, which is always associated with practical implementations of variable structure control, is presented with reference to a class of uncertain multi-input nonlinear systems. The solution procedure relies on the application of an original control approach capable of enforcing a second-order sliding mode (i.e., a sliding regime on a surface s[x(t)]=0 in the system state space, with s/spl dot/[x(t)] identically equal to zero, a regime enforced by a control signal depending on s[x(t)], but directly acting only on s/spl uml/[x(t)]). Such an approach, in its original formulation, only applies to single-input nonlinear systems with particular types of uncertainties. In the present paper, its validity is extended to multi-input nonlinear systems characterized by uncertainties of more general nature, covering a wide class of real processes.