A genetic locus targeted to the nuclear periphery in living cells maintains its transcriptional competence
TLDR
An inducible system to target a genetic locus to the nuclear lamina in living mammalian cells using three-dimensional time-lapse microscopy is presented, determining that targeting of the locus requires passage through mitosis.Abstract:
The peripheral nuclear lamina, which is largely but not entirely associated with inactive chromatin, is considered to be an important determinant of nuclear structure and gene expression. We present here an inducible system to target a genetic locus to the nuclear lamina in living mammalian cells. Using three-dimensional time-lapse microscopy, we determined that targeting of the locus requires passage through mitosis. Once targeted, the locus remains anchored to the nuclear periphery in interphase as well as in daughter cells after passage through a subsequent mitosis. Upon transcriptional induction, components of the gene expression machinery are recruited to the targeted locus, and we visualized nascent transcripts at the nuclear periphery. The kinetics of transcriptional induction at the nuclear lamina is similar to that observed at an internal nuclear region. This new cell system provides a powerful approach to study the dynamics of gene function at the nuclear periphery in living cells.read more
Citations
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Domain organization of human chromosomes revealed by mapping of nuclear lamina interactions
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TL;DR: A high-resolution map of the interaction sites of the entire genome with NL components in human fibroblasts is constructed and demonstrates that the human genome is divided into large, discrete domains that are units of chromosome organization within the nucleus.
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Chromatin architecture reorganization during stem cell differentiation
Jesse R. Dixon,Inkyung Jung,Siddarth Selvaraj,Yin Shen,Jessica Antosiewicz-Bourget,Ah Young Lee,Zhen Ye,Audrey Kim,Nisha Rajagopal,Wei Xie,Yarui Diao,Jing Liang,Huimin Zhao,Victor V. Lobanenkov,Joseph R. Ecker,James A. Thomson,Bing Ren +16 more
TL;DR: Mapping genome-wide chromatin interactions in human embryonic stem cells and four human ES-cell-derived lineages reveals extensive chromatin reorganization during lineage specification, providing a global view of chromatin dynamics and a resource for studying long-range control of gene expression in distinct human cell lineages.
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Charting histone modifications and the functional organization of mammalian genomes
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TL;DR: A selection of recent studies that have probed histone modifications and successive layers of chromatin structure in mammalian genomes, the patterns that have been identified and future directions for research are reviewed.
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Molecular Maps of the Reorganization of Genome-Nuclear Lamina Interactions during Differentiation
Daan Peric-Hupkes,Wouter Meuleman,Wouter Meuleman,Ludo Pagie,Sophia W.M. Bruggeman,Irina Solovei,Wim Brugman,Stefan Gräf,Paul Flicek,Ron M. Kerkhoven,Maarten van Lohuizen,Marcel J. T. Reinders,Lodewyk F. A. Wessels,Lodewyk F. A. Wessels,Bas van Steensel +14 more
TL;DR: High-resolution maps of genome-nuclear lamina interactions during subsequent differentiation of mouse embryonic stem cells via lineage-committed neural precursor cells into terminally differentiated astrocytes suggest that lamina-genome interactions are widely involved in the control of gene expression programs during lineage commitment and terminal differentiation.
Journal ArticleDOI
Nuclear lamins: major factors in the structural organization and function of the nucleus and chromatin
Thomas Dechat,Katrin Pfleghaar,Kaushik Sengupta,Takeshi Shimi,Dale K. Shumaker,Liliana Solimando,Robert D. Goldman +6 more
TL;DR: An up-to-date overview of the functions of nuclear lamins is provided, emphasizing their roles in epigenetics, chromatin organization, DNA replication, transcription, and DNA repair.
References
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