Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Improvement of enzyme activity, stability and selectivity via immobilization techniques
Cesar Mateo,Jose M. Palomo,Gloria Fernández-Lorente,Jose M. Guisan,Roberto Fernandez-Lafuente +4 more
TL;DR: In all cases, enzyme engineering via immobilization techniques is perfectly compatible with other chemical or biological approaches to improve enzyme functions and the final success depend on the availability of a wide battery of immobilization protocols.
Journal ArticleDOI
Atomic structure of acetylcholinesterase from Torpedo californica: a prototypic acetylcholine-binding protein
Joel L. Sussman,Michal Harel,Felix Frolow,Christian Oefner,Adrian Goldman,Lilly Toker,Israel Silman +6 more
TL;DR: Modeling of acetylcholine binding to the enzyme suggests that the quaternary ammonium ion is bound not to a negatively charged "anionic" site, but rather to some of the 14 aromatic residues that line the gorge.
Journal ArticleDOI
Metabolic Engineering of Poly(3-Hydroxyalkanoates): From DNA to Plastic
Lara Madison,Gjalt W. Huisman +1 more
TL;DR: An overview of the different PHA biosynthetic systems and their genetic background is provided, followed by a detailed summation of how this natural diversity is being used to develop commercially attractive, recombinant processes for the large-scale production of PHAs.
Journal ArticleDOI
Molecular and cellular biology of cholinesterases
TL;DR: In this article, the authors propose a method to identify the root cause of a problem.Abbreviations: [2]... ].., [3]
References
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Journal ArticleDOI
Rhizomucor miehei triglyceride lipase is synthesized as a precursor.
TL;DR: Conservation of an intron in an isolated cDNA recombinant and immunoprecipitation of in vitro synthesized R. miehei translation products indicates that the expression of the lipase gene might involve inefficient mRNA splicing.
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Complete nucleotide sequence of the lipase gene from Staphylococcus hyicus cloned in Staphylococcus carnosus
TL;DR: DNA sequencing has revealed the location of the gene, the ribosomal binding site and the presence of a typical signal sequence from the lipase gene from Staphylococcus hyicus subsp.
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Partial purification and characterization of free and immobilized lipases fromMucor miehei
TL;DR: The results indicate that the B lipase is formed by partial deglycosylation of the A lipase and that this influences the activity toward emulsions, and the two enzymes have been immobilized by adsorption.
Journal ArticleDOI
Molecular cloning and nucleotide sequence of rat lingual lipase cDNA
Andrew J. P. Docherty,M.W. Bodmer,S. Angal,Robert Verger,C. Rivière,P.A. Lowe,A. Lyons,John Spencer Emtage,T.J.R. Harris +8 more
TL;DR: Purified rat lingual lipase (EC3113), a glycoprotein of approximate molecular weight 52,000, was used to generate polyclonal antibodies which were able to recognise the denatured and deglycosylated enzyme and screen a cDNA library prepared from mRNA isolated from the serous glands of rat tongue cloned in E. coli expression vectors.
Journal ArticleDOI
Rhizomucor miehei triglyceride lipase is processed and secreted from transformedAspergillus oryzae
Birgitte Huge-Jensen,Frank Andreasen,Tove Christensen,Mogens Christensen,Lars Thim,Esper Boel +5 more
TL;DR: The utility of this organism as a host for the production of recombinant triglyceride lipases is demonstrated and it is demonstrated that this organism has the same characteristics with respect to mobility on reducing SDS-gels and amino acid composition as the native enzyme.