Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Journal ArticleDOI
Correlation of the absolute conformation of sec alcohols derived from macrocyclic lactones of resorcylic acid and their stereoselective transacetylation in organic solvents by Pseudomonas fluorescens lipase
TL;DR: In this paper, the structural and conformational properties of 7α and 7β isomers have been determined by X-ray analysis and the importance of conformational chirality in the hydrophobic region of these substrates for stereoselection in transacylation by microbial lipases is discussed.
Journal ArticleDOI
Influence of structural factors and enzyme type on the reactivity and enantioselectivity of the enzymatic esterification of bicyclic meso dialcohols
TL;DR: In this article, the meso dialcohols 1-7, obtained from the Diels-Alder adducts furan/maleic anhydride, and were subjected to enzymatic esterification in organic solvents.
Patent
Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
TL;DR: In this paper, a method for the production of a lipid acyltransferase comprising the steps of: (i) providing a Bacillus licheniformis cell, (ii) transforming the BCL cell with a heterologous nucleotide sequence encoding a lipid, and (iii) expressing the lipid in the cell under the control of a promoter sequence.
Patent
Crystal structure of monoacylglycerol lipase (mgll)
Carsten Schubert,Bruce L. Grasberger,Celine Schalk-Hihi,Diane M. Maguire,Frank A. Lewandowski,Cynthia M. Milligan,Richard Alexander +6 more
TL;DR: A number of soluble engineered forms of MGLL that are suitable for high-throughput screening and protein crystallization, as well as a crystallized form of monoacylglycerol lipase protein (MGLL) and descriptions of the X-ray diffraction patterns are disclosed in this article.
References
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Journal ArticleDOI
A graphics model building and refinement system for macromolecules
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TL;DR: Some extensions of the current export versions of the programs that have been implemented or are envisioned are described, expected to be important in realizing the goal of producing refined structural models that reproduce the diffraction patterns to within the accuracy of the measured data and which are compatible with prior stereochemical knowledge of macromolecules.
Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.