Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Complicated effects of a zwitterionic polymer containing dimethyl chains on the structures, activities and stabilities of different enzymes
TL;DR: The complicated effects of a zwitterionic polymer containing dimethyl chains (pID) on the activity, stability and structure of three different enzymes, i.e., Candida rugosa lipase, lysozyme and catalase are reported via activity assays and spectroscopic analysis.
Journal ArticleDOI
Michaelis–Menten Kinetics for Enzymatic Depolymerization of a Linear Polyester Synthesized from Z-Protected Glutamic Acid
TL;DR: A biodegradable polyester resin was polymerized from N-benzyloxycarbonyl-L-glutamic acid and ethylene glycol and Rhizopus delemar lipase was used as a biocatalyst for the rupture of ester bonds during the hydrolysis studies.
Journal ArticleDOI
A new lipase (Alip2) with high potential for enzymatic hydrolysis of the diester diethyladipate to the monoester monoethyladipate.
TL;DR: In this paper, several putative lipase genes from the genome of the yeast Blastobotrys (Arxula) raffinosifermentans (adeninivorans) LS3 were overexpressed in the yeast itself and screened for the desymmetrization of the dicarboxylic acid diester diethyl adipate into the monoester monoethyl adipate (MEA), which can serve as a monomeric spacer group for functional polymers used in medical chemistry and dental applications.
Journal ArticleDOI
Crystallization and preliminary X-ray crystallographic analysis of arylesterase from Vibrio mimicus.
TL;DR: Single crystals of arylesterase from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months and belong to monoclinic space group P21.
Journal Article
Double Immobilized Lipase for the Kinetic Resolution of Secondary Alcohols
TL;DR: A new and improved biocatalyst was obtained by deposition of the entrapped lipase on a solid support, which can be used with excellent results in acylation reactions.
References
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Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.