Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Acetyl xylan esterase from trichoderma reesei contains an active-site serine residue and a cellulose-binding domain
TL;DR: The axe1 gene encoding acetyl xylan esterase was isolated from an expression library of the filamentous fungus Trichoderma reesei using antibodies raised against the purified enzyme, which has a clear similarity with fungal cutinases which are serine esterases.
Journal ArticleDOI
Functional sites in protein families uncovered via an objective and automated graph theoretic approach.
TL;DR: The DRESPAT method does not require a user-defined pattern, size or location of the pattern and therefore, has the potential to uncover new functional sites in protein families.
Journal ArticleDOI
A pancreatic lipase with a phospholipase A1 activity: crystal structure of a chimeric pancreatic lipase-related protein 2 from guinea pig.
Chrislaine Withers-Martinez,Frédéric Carrière,Robert Verger,Dominique Bourgeois,Christian Cambillau +4 more
TL;DR: The structures of the related enzymes, GPLRP2 and HPL and the model of Dolml PLA1, provide insights into the role played by the loops located above the active site in controlling substrate selectivity towards triglycerides or phospholipids.
Journal ArticleDOI
Entrapment of lipases in hydrophobic magnetite-containing sol-gel materials: magnetic separation of heterogeneous biocatalysts
TL;DR: In this paper, the authors showed that the simultaneous entrapment of a lipase such as Pseudomonas cepacia or Candida antarctica and nanostructured superparamagnetic magnetite (Fe3O4) in hydrophobic sol-gel materials derived from CH3Si(OCH3)3 (MTMOS) or other hydrophilic precursors leads to catalytically active, mechanically stable and magnetically separable heterogeneous biocatalysts.
Journal ArticleDOI
Identification of the catalytic triad of the lipase/acyltransferase from Aeromonas hydrophila.
M J Brumlik,J T Buckley +1 more
TL;DR: Aeromonas hydrophila secretes a lipolytic enzyme that has several properties in common with the mammalian enzyme lecithin-cholesterol acyltransferase, and it is concluded that Asp-116 and His-291 compose the catalytic triad of the enzyme.
References
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TL;DR: An analysis of the solvent content of 116 different crystal forms of globular proteins found that in many cases this range will be sufficiently restrictive to enable the probable number of molecules in the crystallographic asymmetric unit to be determined directly from the molecular weight of the protein and the space group and unit cell dimensions of the crystal.
Journal ArticleDOI
A graphics model building and refinement system for macromolecules
TL;DR: A model building and refinement system is described for use with a Vector General 3400 display that has been used to assist in difference Fourier map interpretation at medium and high resolution, and to build a protein molecule into a multiple isomorphous replacement phased electron density map.
Book ChapterDOI
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TL;DR: Some extensions of the current export versions of the programs that have been implemented or are envisioned are described, expected to be important in realizing the goal of producing refined structural models that reproduce the diffraction patterns to within the accuracy of the measured data and which are compatible with prior stereochemical knowledge of macromolecules.
Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.