Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Trp89 in the lid of Humicola lanuginosa lipase is important for efficient hydrolysis of tributyrin.
Mats Holmquist,Mats Martinelle,Ib Groth Clausen,Shamkant Anant Patkar,Allan Svendsen,Karl Hult +5 more
TL;DR: The study showed that Trp89 located in the lid of H. lanuginosa lipase is important for the efficient hydrolysis of tributyrin and that this residue plays a role in the catalytic steps after adsorption of the lipase to the substrate interface.
Journal ArticleDOI
Tailoring mesoporous-silica nanoparticles for robust immobilization of lipase and biocatalysis
Mohammad Kalantari,Meihua Yu,Yannan Yang,Ekaterina Strounina,Zhengying Gu,Xiaodan Huang,Jun Zhang,Hao Song,Chengzhong Yu +8 more
TL;DR: It is demonstrated that the increased hydrophobic content and a tailored pore size (slightly larger than the size of lipase) are responsible for the high performance of immobilized lipase.
Journal ArticleDOI
Functional expression of a tobacco gene related to the serine hydrolase family Esterase activity towards short-chain dinitrophenyl acylesters
TL;DR: Sequence similarities between the tobacco esterase and expressed sequence tags in databases suggest the existence of members of this enzyme family in various plant species.
Journal ArticleDOI
Structural relationship between lipases and peptidases of the prolyl oligopeptidase family.
TL;DR: A comparison of the relative positions of the three catalytic residues in peptidases and microbial lipases indicated structural and possibly evolutionary relationship between the two families and identified the catalytic Asp residue in the prolyl oligopeptidase family.
Journal ArticleDOI
Immobilized lipases for biodiesel production: Current and future greening opportunities
TL;DR: This review presents a way forward to address immobilized lipase cost by using green chemistry strategies that have shown success in recent studies to supplant chemical catalysts currently used in biodiesel production.
References
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Journal ArticleDOI
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Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.