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Journal ArticleDOI

A serine protease triad forms the catalytic centre of a triacylglycerol lipase.

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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.
Abstract
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.

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Citations
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Journal ArticleDOI

Interactions between a lipase and charged surfactants--a comparison between bulk and interfaces.

TL;DR: The difference in behavior of the system cationic surfactant-lipase in bulk and at the interfaces may be due to the change in enzyme conformation that is known to occur at interfaces between water and an apolar phase.
Journal ArticleDOI

The novel hexadecyltrimethylammonium bromide (CTAB) based organogel as reactor for ester synthesis by entrapped Candida rugosa lipase

TL;DR: It is found that lipase entrapped in this system is able to catalyze the esterification reaction of pentanol with caprylic acid in hexane, and highly reproducible results and quantitative production of pentyl caprylate was obtained at low costs.
Book ChapterDOI

Phasing on rapidly soaked ions.

TL;DR: The rapid soak approach uses this property of protein crystals that allows small ions to diffuse within a short time to the solvent regions surrounding the protein molecules and adopt ordered sites at their surfaces to solve macromolecular crystal structures.
Journal ArticleDOI

Serine peptidase catalytic machinery: Cooperative one‐step mechanism

TL;DR: Twenty seven transition-state models are investigated at the restricted Hartree–Fock (RHF) level within the minimal MINI-1′ basis set and the intrinsic reactivity of the class-A and class-C β-lactamases are under concern.
Book ChapterDOI

Computational Design of Molecularly Imprinted Polymers

TL;DR: This chapter discusses recent successes in the use of computational design for the development of one particular branch of synthetic receptors – molecularly imprinted polymers.
References
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Journal ArticleDOI

The Protein Data Bank: a computer-based archival file for macromolecular structures.

TL;DR: The Protein Data Bank is a computer-based archival file for macromolecular structures that stores in a uniform format atomic co-ordinates and partial bond connectivities, as derived from crystallographic studies.
Journal ArticleDOI

Solvent content of protein crystals.

TL;DR: An analysis of the solvent content of 116 different crystal forms of globular proteins found that in many cases this range will be sufficiently restrictive to enable the probable number of molecules in the crystallographic asymmetric unit to be determined directly from the molecular weight of the protein and the space group and unit cell dimensions of the crystal.
Journal ArticleDOI

A graphics model building and refinement system for macromolecules

TL;DR: A model building and refinement system is described for use with a Vector General 3400 display that has been used to assist in difference Fourier map interpretation at medium and high resolution, and to build a protein molecule into a multiple isomorphous replacement phased electron density map.
Book ChapterDOI

Stereochemically restrained refinement of macromolecular structures.

TL;DR: Some extensions of the current export versions of the programs that have been implemented or are envisioned are described, expected to be important in realizing the goal of producing refined structural models that reproduce the diffraction patterns to within the accuracy of the measured data and which are compatible with prior stereochemical knowledge of macromolecules.
Journal ArticleDOI

Human lipoprotein lipase complementary DNA sequence

TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.
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