Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Effectiveness Of Ultrafiltration On The Recovery And Reuse Of Liquid Enzymes In The Production Of Biodiesel
TL;DR: In this article, the authors evaluated the use of enzyme catalysts in the production of biodiesel and compared two enzyme recovery techniques of a simple settling method versus ultra-filtration were compared.
Journal Article
Hongos y levaduras: fábricas de lipasas
Ricardo Martínez Corona,Carlos Cortés Penagos,Luis Alberto Madrigal Pérez,Juan Carlos González Hernández +3 more
TL;DR: The general and production characteristics of currently utilized lipases from various fungal microorganisms are covered in order to determine the existing alternatives for the industrial biotechnological processes that demand this type of enzymes.
Journal ArticleDOI
Regio-selective lipase catalyzed hydrolysis of oxanorbornane-based sugar-like amphiphiles at air-water interface: A polarized FT-IRRAS study.
TL;DR: Interfacial hydrolysis of oxanorbornane-based amphiphile (Triol C16) by Candida rugosa lipase was investigated using real-time polarized Fourier transform-infrared reflection absorption spectroscopy (FT-IRRAS) to demonstrate further the potential of IRRAS spectroscopic monitoring the hydrolytic product formation and selectivity at biomimetic interfaces.
Journal ArticleDOI
Substrate-selectivity in acylhydrolases: a cautionary tale
TL;DR: Three cases, where in spite of detailed structural knowledge of the enzymes, their true biological function is far from clear, are discussed, which include platelet-activating factor acetylhydrolases, acyl protein thioesterase I and peroxisomal medium-length chainThioesterases.
Journal ArticleDOI
Combination of Enzymes and Deep Eutectic Solvents as Powerful Toolbox for Organic Synthesis
TL;DR: In this article , a review aims to show the newly reported protocols in the field, subdivided by reaction class as a 'toolbox' guide for organic synthesis, as an improvement in terms of selectivity and sustainability.
References
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A graphics model building and refinement system for macromolecules
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Book ChapterDOI
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Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.