Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Biochemical characterization of a new thermostable lipase from Bacillus pumilus strain
Faouzi Laachari Faouzi,Fatimazahra El Bergadi,Adel Sayari,Soumya Elabed,Iraqui Mohammed Mohammed,El Hassan Harchali Harchali,Saad Koraichi Ibnsouda +6 more
TL;DR: This newly isolated lipase from bacterial strains isolated from tannery waters in the old medina of Fez is thermostable and it has a significant difference which was observed when the biochemical properties of the Bacillus pumilus lipase were compared to others microbial lipases.
Journal ArticleDOI
Medium - engineering: a useful tool for modulating lipase activity and selectivity
TL;DR: The key features of lipases are reviewed and how medium-engineering is a useful tool to modulate the activity and selectivity of lipase-catalyzed reactions is demonstrated.
Journal ArticleDOI
Impact of wheat endogenous lipids on the quality of fresh bread: Key terms, concepts, and underlying mechanisms.
Sara Melis,Jan A. Delcour +1 more
TL;DR: The effects that wheat endogenous lipids and their enzymatically released hydrolysis products have on fresh bread properties and the mechanisms whereby they exert these effects are reviewed.
Journal ArticleDOI
Lipase-Catalyzed Regioselective Acylation and Deacylation of Glucose Derivatives
TL;DR: The lipase from Candida antarctica proved particularly efficient for catalyzing selective hydrolysis in the 1-position of 1,2,3,4,6-penta-O-acetyl-β-D-glucose (β-1), thereby providing an efficient chemo-enzymatic synthesis starting from readily available raw materials.
Journal ArticleDOI
Molecular Mechanism behind Solvent Concentration-Dependent Optimal Activity of Thermomyces lanuginosus Lipase in a Biocompatible Ionic Liquid: Interfacial Activation through Arginine Switch.
TL;DR: Molecular dynamics simulations of TLL in aqueous solutions of a biocompatible ionic liquid (IL), cholinium glycinate (ChGly), have been carried out to investigate the microscopic reasons for the experimentally observed enhancement in the activity of T LL upon addition of a room temperature IL (RTIL), especially at an optimal concentration.
References
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Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.