Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Book ChapterDOI
Immobilization of Lipases by Adsorption on Hydrophobic Supports: Modulation of Enzyme Properties in Biotransformations in Anhydrous Media.
TL;DR: Methodology of immobilization into hydrophobic carriers is described using as case study the preparation of immobilized biocatalysts of Thermomyces lanuginosus lipase (TLL), and the following particular features will be discussed.
Journal ArticleDOI
Molecular cloning and characterization of rabbit pancreatic triglyceride lipase
TL;DR: High homology in the amino acid sequence and composition is also apparent between rabbit and other species like dog, pig, pig and rat, and highest homology is found to be around active-site serine.
Book ChapterDOI
Probing Conformational Changes and Interfacial Recognition Site of Lipases With Surfactants and Inhibitors
Eduardo Mateos-Diaz,Sawsan Amara,Alain Roussel,Sonia Longhi,Christian Cambillau,Frédéric Carrière +5 more
TL;DR: The mechanism by which surfactants promote the lid opening can be further investigated in solution by site-directed spin labeling of lipase coupled to electron paramagnetic resonance spectroscopy and experimental approaches are illustrated here by results obtained with mammalian digestive lipases, fungal lipase, and cutinases.
Journal ArticleDOI
A continuous fluorescence-displacement assay for triacylglycerol lipase and phospholipase C that also allows the measurement of acylglycerols.
TL;DR: A new continuous fluorescence-displacement assay for enzymes that release long-chain fatty acids is described in detail for pig pancreatic triacylglycerol lipase, allowing the expression of full activity with low concentrations of substrates in the absence of detergents.
Journal ArticleDOI
Fluorescence study of fungal lipase from humicola lanuginosa
TL;DR: The results suggest that distinct configurations of fungal lipase can be monitored using the fluorescence of the Trp89 residue located in the ‘lid’-helix which participates in an interfacial activation of the enzyme.
References
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Journal ArticleDOI
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Journal ArticleDOI
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TL;DR: An analysis of the solvent content of 116 different crystal forms of globular proteins found that in many cases this range will be sufficiently restrictive to enable the probable number of molecules in the crystallographic asymmetric unit to be determined directly from the molecular weight of the protein and the space group and unit cell dimensions of the crystal.
Journal ArticleDOI
A graphics model building and refinement system for macromolecules
TL;DR: A model building and refinement system is described for use with a Vector General 3400 display that has been used to assist in difference Fourier map interpretation at medium and high resolution, and to build a protein molecule into a multiple isomorphous replacement phased electron density map.
Book ChapterDOI
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TL;DR: Some extensions of the current export versions of the programs that have been implemented or are envisioned are described, expected to be important in realizing the goal of producing refined structural models that reproduce the diffraction patterns to within the accuracy of the measured data and which are compatible with prior stereochemical knowledge of macromolecules.
Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.