Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Effect of water, organic solvent and adsorbent contents on production of biodiesel fuel from canola oil catalyzed by various lipases immobilized on epoxy-functionalized silica as low cost biocatalyst
TL;DR: In this article, the effect of water, t-butanol and water adsorbent content on the yield of fatty acid methyl ester (FAME) was considered for the enzymatic production of biodiesel from canola oil using self-made covalently immobilized lipase from Candida antarctica (CALB), TLL and RML.
Journal ArticleDOI
Quantitative determination of intracellular depolymerase activity in Pseudomonas oleovorans inclusions containing poly‐3‐hydroxyalkanoates with long alkyl substituents
TL;DR: Poly-3-hydroxy-octanoate granules isolated from Pseudomonas oleovorans, which were incubated at 30 degrees C in an alkaline buffer, exhibited a linear degradation rate and the depolymerase was demonstrated to be associated with the polymer granule complex and most likely possessed serine residues at its active site.
Journal ArticleDOI
A lux-specific myristoyl transferase in luminescent bacteria related to eukaryotic serine esterases.
Stefano Ferri,Edward A. Meighen +1 more
TL;DR: Analysis of the substrate specificities for fatty acyl-ACPs of the transferases from divergent luminescent bacteria has demonstrated that myristoyl-ACP is cleaved at the highest rate and supports a common metabolic role for both enzymes in the diversion of fatty acids from the fatty acid biosynthetic pathway.
Journal ArticleDOI
Lipase immobilization on modified zirconia nanoparticles: Studies on the effects of modifiers
TL;DR: Zirconia nanoparticles modified with carboxylic acid with a long alkyl chain can significantly enhance the activity of immobilized lipases used for asymmetric synthesis in organic media as mentioned in this paper.
Journal ArticleDOI
Probing the substrate specificity for lipases. II. Kinetic and modeling studies on the molecular recognition of 2-arylpropionic esters by Candida rugosa and Rhizomucor miehei lipases.
TL;DR: Racemic arylpropionic esters 1-3, precursors of therapeutically important non-steroidal antiinflammatory drugs, were subjected to hydrolyses in the presence of either Candida rugosa or Rhizomucor miehei crude lipases and proved to be highly enantioselective, whereas 3 was not transformed at all.
References
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Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.