Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Study of lipase immobilization on zeolitic support and transesterification reaction in a solvent free-system
Anastasia Macario,Girolamo Giordano,Leonardo Setti,Attilio Parise,Juan M. Campelo,José M. Marinas,Diego Luna +6 more
TL;DR: The RML immobilized on zeolite materials has been studied and shows a high oleic acid conversion for several reaction cycles with a higher total biodiesel productivity compared to that using the free enzyme.
Journal ArticleDOI
Elucidation of the 4-Hydroxyacetophenone Catabolic Pathway in Pseudomonas fluorescens ACB
Mariëlle J. H. Moonen,Nanne M. Kamerbeek,Adrie H. Westphal,Sjef Boeren,Dick B. Janssen,Marco W. Fraaije,Willem J. H. van Berkel +6 more
TL;DR: Evidence is provided that hydroquinone is further degraded through 4-hydroxymuconic semialdehyde and maleylacetate to beta-ketoadipate and it is proposed that the hapC and hapD gene products are involved in the ring cleavage of Hydroquinone.
Journal ArticleDOI
Bacterial lipolysis of immune-activating ligands promotes evasion of innate defenses.
Xi Chen,Francis Alonzo +1 more
TL;DR: It is demonstrated that a secreted lipase of Staphylococcus aureus also facilitates immune evasion by inactivating bacterial-derived lipoproteins, a pathogen-associated molecular pattern required for innate immune activation.
Journal ArticleDOI
Mutational analysis of a feruloyl esterase from Aspergillus awamori involved in substrate discrimination and pH dependence
Takuya Koseki,Kenji Takahashi,Shinya Fushinobu,Haruyuki Iefuji,Kimio Iwano,Katsumi Hashizume,Hiroshi Matsuzawa +6 more
TL;DR: Kinetic analysis of the mutant enzymes showed that the higher catalytic efficiency of the D77I and Y80F mutants towardalpha-naphthylbutyrate (C4) and alpha-nAPHthylcaprylate (C8), respectively, was due to a lower K(m) value.
Journal ArticleDOI
Mutational and proteolytic studies on a flexible loop in glutathione synthetase from Escherichia coli B: the loop and arginine 233 are critical for the catalytic reaction
TL;DR: The results suggest that the loop covers the active site while ATP and gamma-Glu-Cys bind there and that it protects the unstable gamma-L-glutamyl- L-cysteine phosphate intermediate from decomposition by bulk water.
References
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Journal ArticleDOI
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Book ChapterDOI
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Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.