Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Journal ArticleDOI
Lipase−Surfactant Interactions Studied by Neutron Reflectivity and Ellipsometry
TL;DR: In this paper, the interaction between a microbial lipase and an anionic and a cationic surfactant at the air−water interface has been studied by neutron reflectivity.
Journal ArticleDOI
Diols as substrates in lipase-catalyzed enantioselective acylations - a brief review
TL;DR: The application of lipase-catalyzed enantioselective acylations of diols in organic solvents as a special type of bifunctional compounds as substrates is directed on the synthesis of enantiomerically pure building blocks.
Journal ArticleDOI
DNA cleavage function of seryl-histidine dipeptide and its application
TL;DR: The double-stranded DNA or circular plasmid DNA can be cleaved by the Ser-His dipeptide by hydrolysis of the DNA-phospho-diester bond and the proper sequential order of the amino acids serine and histidine is apparently crucial in its unique cleavage activity.
Book ChapterDOI
The Kinetics, Specificities and Structural Features of Lipases
Stéphane Ransac,Frédéric Carrière,Ewa Rogalska,Robert Verger,Frank Marguet,Gérard Buono,Eduardo P. Melo,Joaquim M. S. Cabral,Marie-Pierre Egloff,Herman van Tübeurgh,Christian Cambillau +10 more
TL;DR: The four main classes of biological substances are carbohydrates, proteins, nucleic acids and lipids as mentioned in this paper, which are all insoluble in water but soluble in apolar and slightly polar solvents such as ether, chloroform and benzene.
Journal ArticleDOI
Ultrasound coating of polydimethylsiloxanes with antimicrobial enzymes
Anat Lipovsky,Barbara Thallinger,Ilana Perelshtein,Roland Ludwig,Christoph Sygmund,Gibson S. Nyanhongo,Georg M. Guebitz,Aharon Gedanken,Aharon Gedanken +8 more
TL;DR: The antimicrobial hydrogen peroxide producing enzyme cellobiose dehydrogenase (CDH) was for the first time grafted onto polydimethylsiloxanes (PDMS) using an ultrasound assisted coating method to develop an effective in situ continous H2O2 producing system to continuously prevent microbial colonization and biofilm formation on catheters.
References
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Journal ArticleDOI
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Frances C. Bernstein,Thomas F. Koetzle,Graheme J. B. Williams,Edgar F. Meyer,Michael D. Brice,John R. Rodgers,O. Kennard,Takehiko Shimanouchi,Mitsuo Tasumi +8 more
TL;DR: The Protein Data Bank is a computer-based archival file for macromolecular structures that stores in a uniform format atomic co-ordinates and partial bond connectivities, as derived from crystallographic studies.
Journal ArticleDOI
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TL;DR: An analysis of the solvent content of 116 different crystal forms of globular proteins found that in many cases this range will be sufficiently restrictive to enable the probable number of molecules in the crystallographic asymmetric unit to be determined directly from the molecular weight of the protein and the space group and unit cell dimensions of the crystal.
Journal ArticleDOI
A graphics model building and refinement system for macromolecules
TL;DR: A model building and refinement system is described for use with a Vector General 3400 display that has been used to assist in difference Fourier map interpretation at medium and high resolution, and to build a protein molecule into a multiple isomorphous replacement phased electron density map.
Book ChapterDOI
Stereochemically restrained refinement of macromolecular structures.
TL;DR: Some extensions of the current export versions of the programs that have been implemented or are envisioned are described, expected to be important in realizing the goal of producing refined structural models that reproduce the diffraction patterns to within the accuracy of the measured data and which are compatible with prior stereochemical knowledge of macromolecules.
Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.