Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Journal ArticleDOI
Lipases at interfaces: unique interfacial properties as globular proteins.
Pedro Reis,Reinhard Miller,Jürgen Krägel,Martin E. Leser,V. B. Fainerman,Heribert Watzke,Krister Holmberg +6 more
TL;DR: The results indicate that the understanding of the physical properties of lipases can lead to novel approaches to regulate their activity, and contributes to a better understanding of lipase interfacial activation and the mechanisms regulating lipolysis.
Journal ArticleDOI
Structure of Human Pancreatic Lipase-Related Protein 2 with the Lid in an Open Conformation
Cécilia Eydoux,Silvia Spinelli,Tara L. Davis,John R. Walker,Alma Seitova,Sirano Dhe-Paganon,Alain De Caro,Christian Cambillau,Frédéric Carrière +8 more
TL;DR: It is shown that the HPLRP2 is directly inhibited by E600, does not present interfacial activation, and acts preferentially on substrates forming monomers or small aggregates (micelles) dispersed in solution like monoglycerides, phospholipid and galactolipids, whereas classical PL displays reverse properties and a high specificity for unsoluble substrates like triglycerides and diglycerides forming oil-in-water interfaces.
Journal ArticleDOI
The multifunctional role of hormone-sensitive lipase in lipid metabolism.
TL;DR: A finding unexplained for many years has been that, although lipolysis can be stimulated 50-100-fold in adipocytes by lipolytic hormones, HSL can apparently only be activated 2-3-fold via phosphorylation in vitro by cyclic AMP-dependent protein kinase.
Journal ArticleDOI
Volume holographic imaging in transmission geometry
TL;DR: It is shown that appropriately designed objective optics can significantly improve the depth resolution or the working distance of plane-wave reference holographic imaging systems.
Journal ArticleDOI
Purification and characterization of mono- and diacylglycerol lipase isolated from Penicillium camembertii U-150
Shotaro Yamaguchi,Tamio Mase +1 more
TL;DR: A novel enzyme hydrolysing mono- and diacylglycerol was found in the culture filtrate of an isolated fungus, Penicillium camembertii, which was separated into two forms with almost the same molecular weight, amino acid composition and identical N-terminal amino acid sequence.
References
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Journal ArticleDOI
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Book ChapterDOI
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Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.