Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Journal ArticleDOI
New insights into the molecular mechanism of methanol-induced inactivation of Thermomyces lanuginosus lipase: a molecular dynamics simulation study
TL;DR: Detailed molecular dynamics simulations of Thermomyces lanuginosus lipase in water and methanol and compared the observed structural and dynamic properties highlight the importance of surface residue redesign to improve the stability of lipases in meethanol environments.
Journal ArticleDOI
Estimation of the lipase PS (Pseudomonas cepacia) active site dimensions based on molecular mechanics calculations
TL;DR: The proposed model has been based on secondary alcohols, which are efficiently enantiodifferentiated by the enzyme as substrates and accounts for the enantioselectivity shown by the enzymes in transesterification reactions ofsecondary alcohols.
Journal ArticleDOI
Regulation of the interfacial activation within the Candida rugosa lipase family
TL;DR: In this article, the 3D structure of three lipase isoenzymes was solved (Lip1, Lip2 and Lip3) and the fungus Candida rugosa produces several lipase enzymes.
Book ChapterDOI
Lipase-surfactant interactions
TL;DR: In this article, the authors investigated the interaction between different amphiphiles and Rhizomucor miehei lipase by a variety of techniques, including surface tension measurements and ellipsometry.
Journal ArticleDOI
Cold Active Lipases: Biocatalytic Tools for Greener Technology.
TL;DR: In this paper, the authors present an update on cold active lipases from microbial sources along with some structural features justifying high enzyme activity at low temperature and discuss recent achievements on their use in various industries.
References
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Frances C. Bernstein,Thomas F. Koetzle,Graheme J. B. Williams,Edgar F. Meyer,Michael D. Brice,John R. Rodgers,O. Kennard,Takehiko Shimanouchi,Mitsuo Tasumi +8 more
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Journal ArticleDOI
Solvent content of protein crystals.
TL;DR: An analysis of the solvent content of 116 different crystal forms of globular proteins found that in many cases this range will be sufficiently restrictive to enable the probable number of molecules in the crystallographic asymmetric unit to be determined directly from the molecular weight of the protein and the space group and unit cell dimensions of the crystal.
Journal ArticleDOI
A graphics model building and refinement system for macromolecules
TL;DR: A model building and refinement system is described for use with a Vector General 3400 display that has been used to assist in difference Fourier map interpretation at medium and high resolution, and to build a protein molecule into a multiple isomorphous replacement phased electron density map.
Book ChapterDOI
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TL;DR: Some extensions of the current export versions of the programs that have been implemented or are envisioned are described, expected to be important in realizing the goal of producing refined structural models that reproduce the diffraction patterns to within the accuracy of the measured data and which are compatible with prior stereochemical knowledge of macromolecules.
Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.