Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Lipoprotein lipases and vitellogenins in relation to the known three-dimensional structure of pancreatic lipase
TL;DR: A 106‐residue region of high similarity between lipoprotein/pancreatic/hepatic lipases and Drosophila vitellogenins encompasses four β‐strands with all residues but one strictly conserved or conservatively replaced between the structures, and enclosing the putative active site Ser‐152.
Journal ArticleDOI
High-level heterologous expression of an alkaline lipase gene from Penicillium cyclopium PG37 in Pichia pastoris
TL;DR: A 777-bp cDNA fragment encoding a mature alkaline lipase (LipI) from Penicillium cyclopium PG37 was amplified by RT–PCR, and inserted into the expression plasmid pPIC9 K, resulting in the integration of the gene LipI into the P. pastoris GS115 genome.
Journal ArticleDOI
Asymmetric syntheses of nitroalkanols using Pseudomonas sp. lipase: a proposal for the selection of the solvent system of lipase-catalyzed transesterification
Takashi Kitayama,Tomoko Rokutanzono,Ryuhei Nagao,Yuji Kubo,Masahiro Takatani,Kaoru Nakamura,Tadashi Okamoto +6 more
TL;DR: By lipase-catalyzed stereoselective transesterification using Amano AK from Pseudomonas sp.
Patent
Methods of producing 7-carbon chemicals via carbon chain elongation associated with cyclohexane carboxylate synthesis
TL;DR: In this paper, the authors describe biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid and 7-hydroxyheptanic acid from Syntrophus aciditrophicus.
Journal ArticleDOI
Purification, biochemical and molecular study of lipase producing from a newly thermoalkaliphilic Aeribacillus pallidus for oily wastewater treatment.
Ameni Ktata,Najeh Krayem,Ahmed Aloulou,Sofiane Bezzine,Adel Sayari,Mohamed Chamkha,Aida Karray +6 more
TL;DR: The deduced amino acids sequence of the GPL shows an important level of identity with Geobacillus lipases, and displayed an important resistance at high temperature, pH, NaCl, at the presence of detergents and organic solvents.
References
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Journal ArticleDOI
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