Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Dissertation
Production Of Novel Lipase Inhibitor From Streptomyces Sp
TL;DR: The present work is an attempt to screen, isolate and characterize pancreatic lipase inhibitor from actinomycetes.
Journal ArticleDOI
A highly selective receptor for zwitterionic proline.
Alvaro G Temprano,Laura M. Monleón,Omayra H. Rubio,Luis Simón Rubio,Asunción B. Pérez,Francisca Sanz,Joaquín R. Morán +6 more
TL;DR: A chiral chromane receptor has been synthesized which mimics the oxyanion hole of the enzymes, and H-bonds and cation-π interactions team up to generate an apolar host-guest complex with zwitterionic proline.
Book ChapterDOI
Effects of lipid-borne compounds on the activity and stability of lipases in microaqueous systems for the lipase-catalyzed interesterification
X. Xu,J. Adler-Nissen +1 more
TL;DR: In this paper, the effects of lipid-borne compounds such as lipid hydroperoxides, lipid polymers, phospholipids and emulsifiers, cholorophyll and carotenoids, tocopherols, citric acid, partial glycerides and free fatty acids on the activity and stability of lipases are discussed.
Book ChapterDOI
Selective Acylation of Sugar Derivatives Catalyzed by Immobilized Lipase
A. T. J. W. De Goede,M. van Oosterom,M. P. J. Van Deurzen,Roger A. Sheldon,H. van Bekkum,F. van Rantwijk +5 more
TL;DR: Alkyl derivatives of glucose, galactose and fructose were acylated by lipase-catalyzed transesterification with alkanoic esters by finding the water activity in the reaction medium had a striking effect on both the rate and the selectivity of the process.
Journal ArticleDOI
Predicting the impact of mutations on the specific activity of Bacillus thermocatenulatus lipase using a combined approach of docking and molecular dynamics
TL;DR: The described study represents a fast and reliable approach to accurately predict the effect of mutations on the activity and selectivity of lipases and also of other enzymes.
References
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Journal ArticleDOI
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TL;DR: The Protein Data Bank is a computer-based archival file for macromolecular structures that stores in a uniform format atomic co-ordinates and partial bond connectivities, as derived from crystallographic studies.
Journal ArticleDOI
Solvent content of protein crystals.
TL;DR: An analysis of the solvent content of 116 different crystal forms of globular proteins found that in many cases this range will be sufficiently restrictive to enable the probable number of molecules in the crystallographic asymmetric unit to be determined directly from the molecular weight of the protein and the space group and unit cell dimensions of the crystal.
Journal ArticleDOI
A graphics model building and refinement system for macromolecules
TL;DR: A model building and refinement system is described for use with a Vector General 3400 display that has been used to assist in difference Fourier map interpretation at medium and high resolution, and to build a protein molecule into a multiple isomorphous replacement phased electron density map.
Book ChapterDOI
Stereochemically restrained refinement of macromolecular structures.
TL;DR: Some extensions of the current export versions of the programs that have been implemented or are envisioned are described, expected to be important in realizing the goal of producing refined structural models that reproduce the diffraction patterns to within the accuracy of the measured data and which are compatible with prior stereochemical knowledge of macromolecules.
Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.