Journal ArticleDOI
A serine protease triad forms the catalytic centre of a triacylglycerol lipase.
Leo Brady,Andrzej M. Brzozowski,Andrzej M. Brzozowski,Zygmunt S. Derewenda,Eleanor J. Dodson,Guy Dodson,S.P. Tolley,Johan P. Turkenburg,Lars Christiansen,Birgitte Huge-Jensen,Leif Norskov,Lars Thim,Ulrich Menge +12 more
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TLDR
The X-ray structure of the Mucor miehei triglyceride lipase is reported and the atomic model obtained reveals a Ser .. His .. Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.Abstract:
True lipases attach triacylglycerols and act at an oil-water interface; they constitute a ubiquitous group of enzymes catalysing a wide variety of reactions, many with industrial potential. But so far the three-dimensional structure has not been reported for any lipase. Here we report the X-ray structure of the Mucor miehei triglyceride lipase and describe the atomic model obtained at 3.1 A resolution and refined to 1.9 A resolution. It reveals a Ser..His..Asp trypsin-like catalytic triad with an active serine buried under a short helical fragment of a long surface loop.read more
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Journal ArticleDOI
Purification of lipases fromChromobacterium viscosum: Is there a high molecular weight lipase?
TL;DR: The results do not completely agree with those reported in the literature, suggesting that the high molecular weight lipase is probably an aggregate of lipases contained in the low molecular weight peak.
Dissertation
Isolement, identification et physiologie des champignons thermophiles en vue de la production de lipases par fermentation en milieu solide
Cordova Lopez,Jesus Antonio +1 more
TL;DR: In this paper, the authors investigate the existence of souches capable of producing thermophiles in the nature of champignons filamenteux capables of se developper a des temperatures elevees and of producing enzymes thermostables with caracteristiques specifiques.
Journal ArticleDOI
Inhibition of Rhizomucor miehei and Candida rugosa lipases by d-glucose in esterification between l-alanine and d-glucose
TL;DR: A detailed kinetic study of the esterification of D-glucose with L-alanine catalyzed by lipases from Rhizomucor miehei and Candida rugosa showed that both lipases follow the Ping-Pong Bi-Bi mechanism, with competitive substrate inhibition by D- glucose at higher concentrations leading to the formation of dead-end lipase.
Journal ArticleDOI
Expression of a thermo-alkaline lipase gene from Talaromyces thermophilus in recombinant Pichia pastoris
Xu Zhang,Xueqi Li,Liming Xia +2 more
TL;DR: This research showed a bright prospect for industrial application of thermo- and alkaline-stable lipase, which was genetically optimized and inserted into the downstream of AOX1 promoter and α-factor to construct recombinant plasmid pPIC9K-TTL, and successfully extracellularly expressed.
Journal ArticleDOI
Characterization of an antigenic serine protease in the Trichinella spiralis adult.
He Gao,Bin Tang,Xue Bai,Libo Wang,Xiu-Ping Wu,Haining Shi,Wang Xuelin,Xiaolei Liu,Mingyuan Liu +8 more
TL;DR: The putatively inactive serine protease-like protein Ts-ADSp-7 could inhibit blood coagulation, and the protein is located in the enlarged nuclei of nurse cells during capsule formation, which might be important molecules in the parasite-host interaction.
References
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Journal ArticleDOI
Human lipoprotein lipase complementary DNA sequence
TL;DR: Analysis of the sequence indicates that human lipoprotein lipase, hepaticlipase, and pancreatic lipase are members of a gene family that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins.