AUREOCHROME1a-Mediated Induction of the Diatom-Specific Cyclin dsCYC2 Controls the Onset of Cell Division in Diatoms (Phaeodactylum tricornutum)
Marie J. J. Huysman,Antonio Emidio Fortunato,Michiel Matthijs,Benjamin Schellenberger Costa,Rudy Vanderhaeghen,Hilde Van den Daele,Matthias Sachse,Dirk Inzé,Chris Bowler,Peter G. Kroth,Christian Wilhelm,Angela Falciatore,Wim Vyverman,Lieven De Veylder +13 more
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It is demonstrated that diatom-specific cyclin 2 (dsCYC2) in Phaeodactylum tricornutum displays a transcriptional peak within 15 min after light exposure, long before the onset of cell division.Abstract:
Cell division in photosynthetic organisms is tightly regulated by light. Although the light dependency of the onset of the cell cycle has been well characterized in various phototrophs, little is known about the cellular signaling cascades connecting light perception to cell cycle activation and progression. Here, we demonstrate that diatom-specific cyclin 2 (dsCYC2) in Phaeodactylum tricornutum displays a transcriptional peak within 15 min after light exposure, long before the onset of cell division. The product of dsCYC2 binds to the cyclin-dependent kinase CDKA1 and can complement G1 cyclin-deficient yeast. Consistent with the role of dsCYC2 in controlling a G1-to-S light-dependent cell cycle checkpoint, dsCYC2 silencing decreases the rate of cell division in diatoms exposed to light-dark cycles but not to constant light. Transcriptional induction of dsCYC2 is triggered by blue light in a fluence rate-dependent manner. Consistent with this, dsCYC2 is a transcriptional target of the blue light sensor AUREOCHROME1a, which functions synergistically with the basic leucine zipper (bZIP) transcription factor bZIP10 to induce dsCYC2 transcription. The functional characterization of a cyclin whose transcription is controlled by light and whose activity connects light signaling to cell cycle progression contributes significantly to our understanding of the molecular mechanisms underlying light-dependent cell cycle onset in diatoms.read more
Citations
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Genome-wide diel growth state transitions in the diatom Thalassiosira pseudonana
Justin Ashworth,Sacha Coesel,Allison Lee,E. Virginia Armbrust,Mónica V. Orellana,Mónica V. Orellana,Nitin S. Baliga +6 more
TL;DR: Repeated shifts in the transcript levels of hundreds of genes encoding sensory, signaling, and regulatory functions accompanied the four cell-state transitions, providing a preliminary map of the highly coordinated gene regulatory program under varying conditions.
Journal ArticleDOI
Diatom Phytochromes Reveal the Existence of Far-Red-Light-Based Sensing in the Ocean
Antonio Emidio Fortunato,Marianne Jaubert,Gen Enomoto,Jean-Pierre Bouly,Raffaella Raniello,Michael Thaler,Shruti Malviya,Juliana S Bernardes,Fabrice Rappaport,Bernard Gentili,Marie J. J. Huysman,Alessandra Carbone,Chris Bowler,Maurizio Ribera d'Alcalà,Masahiko Ikeuchi,Angela Falciatore +15 more
TL;DR: It is shown that two marine diatom species, Phaeodactylum tricornutum and Thalassiosira pseudonana, possess a bona fide red/far-red light sensing phytochrome (DPH) that uses biliverdin as a chromophore and displays accentuated red-shifted absorbance peaks compared with other characterized plant and algal phy tochromes.
Journal ArticleDOI
Transcriptional Orchestration of the Global Cellular Response of a Model Pennate Diatom to Diel Light Cycling under Iron Limitation.
Sarah R. Smith,Sarah R. Smith,Jeroen Gillard,Jeroen Gillard,Adam B. Kustka,John P. McCrow,Jonathan H. Badger,Hong Zheng,Ashley M. New,Christopher L. Dupont,Toshihiro Obata,Alisdair R. Fernie,Andrew E. Allen,Andrew E. Allen +13 more
TL;DR: New insight is provided into transcriptional modulation of diatom physiology and metabolism across light:dark cycles in response to Fe availability, providing mechanistic understanding for the ability of diatoms to remain metabolically poised to respond quickly to Fe input and revealing strategies underlying their ecological success.
Journal ArticleDOI
One-step generation of multiple gene knock-outs in the diatom Phaeodactylum tricornutum by DNA-free genome editing.
Manuel Serif,Gwendoline Dubois,Gwendoline Dubois,Gwendoline Dubois,Anne-Laure Finoux,Anne-Laure Finoux,Anne-Laure Finoux,Marie-Ange Teste,Marie-Ange Teste,Marie-Ange Teste,Denis Jallet,Denis Jallet,Denis Jallet,Fayza Daboussi,Fayza Daboussi,Fayza Daboussi +15 more
TL;DR: A highly efficient multiplex genome-editing method in the diatom Phaeodactylum tricornutum, relying on the biolistic delivery of CRISPR-Cas9 ribonucleoproteins coupled with the identification of two endogenous counter-selectable markers, PtUMPS and PtAPT is reported.
Journal ArticleDOI
Investigating mixotrophic metabolism in the model diatom Phaeodactylum tricornutum
Valeria Villanova,Antonio Emidio Fortunato,Dipali Singh,Davide Dal Bo,Melissa Conte,Toshihiro Obata,Juliette Jouhet,Alisdair R. Fernie,Eric Maréchal,Angela Falciatore,Julien Pagliardini,Adeline Le Monnier,Mark G. Poolman,Gilles Curien,Dimitris Petroutsos,Giovanni Finazzi +15 more
TL;DR: The presence of glycerol, despite provoking features reminiscent of nutrient limitation, neither diminishes photosynthetic activity nor cell growth, revealing essential aspects of the metabolic flexibility of these microalgae and suggesting possible biotechnological applications of mixotrophy.
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