Biowire: a platform for maturation of human pluripotent stem cell-derived cardiomyocytes
Sara S. Nunes,Jason W. Miklas,Jie Liu,Roozbeh Aschar-Sobbi,Yun Xiao,Boyang Zhang,Jiahua Jiang,Stéphane Massé,Mark Gagliardi,Anne Hsieh,Nimalan Thavandiran,Michael A. Laflamme,Kumaraswamy Nanthakumar,Gil J. Gross,Peter H. Backx,Peter H. Backx,Gordon Keller,Milica Radisic +17 more
TLDR
It is demonstrated that the engineered platform allows for the generation of three-dimensional, aligned cardiac tissues (biowires) with frequent striations and that the responses of immature human myocardium to electrical stimulation and pacing are in agreement with cardiomyocyte maturation.Abstract:
Directed differentiation protocols enable derivation of cardiomyocytes from human pluripotent stem cells (hPSCs) and permit engineering of human myocardium in vitro However, hPSC-derived cardiomyocytes are reflective of very early human development, limiting their utility in the generation of in vitro models of mature myocardium Here we describe a platform that combines three-dimensional cell cultivation with electrical stimulation to mature hPSC-derived cardiac tissues We used quantitative structural, molecular and electrophysiological analyses to explain the responses of immature human myocardium to electrical stimulation and pacing We demonstrated that the engineered platform allows for the generation of three-dimensional, aligned cardiac tissues (biowires) with frequent striations Biowires submitted to electrical stimulation had markedly increased myofibril ultrastructural organization, elevated conduction velocity and improved both electrophysiological and Ca(2+) handling properties compared to nonstimulated controls These changes were in agreement with cardiomyocyte maturation and were dependent on the stimulation rateread more
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Advanced maturation of human cardiac tissue grown from pluripotent stem cells
Kacey Ronaldson-Bouchard,Stephen P. Ma,Keith Yeager,Timothy Chen,LouJin Song,Dario Sirabella,Kumi Morikawa,Diogo Teles,Diogo Teles,Masayuki Yazawa,Gordana Vunjak-Novakovic +10 more
TL;DR: Tissue maturity was necessary for achieving physiological responses to isoproterenol and recapitulating pathological hypertrophy, supporting the utility of this tissue model for studies of cardiac development and disease.
Journal ArticleDOI
Engineering Adolescence Maturation of Human Pluripotent Stem Cell–Derived Cardiomyocytes
TL;DR: Progress in promoting the maturation of the hPSC cardiomyocytes is discussed, in the context of the current knowledge of developmental cardiac maturation and in relation to in vitro model systems such as rodent ventricular myocytes.
Journal ArticleDOI
Advances in organ-on-a-chip engineering
TL;DR: This Review examines how tissue barrier properties, parenchymal tissue function and multi-organ interactions can be recreated in organ-on-a-chip systems and applied for drug screening.
Journal ArticleDOI
Bioprinting 3D microfibrous scaffolds for engineering endothelialized myocardium and heart-on-a-chip.
Yu Shrike Zhang,Yu Shrike Zhang,Yu Shrike Zhang,Andrea Arneri,Simone Bersini,Su Ryon Shin,Kai Zhu,Zahra Goli-Malekabadi,Julio Aleman,Cristina Colosi,Fabio Busignani,Valeria Dell’Erba,Colin E. Bishop,Thomas Shupe,Danilo Demarchi,Matteo Moretti,Marco Rasponi,Mehmet R. Dokmeci,Anthony Atala,Ali Khademhosseini +19 more
TL;DR: It is demonstrated that the method for generation of endothelialized organoids fabricated through an innovative 3D bioprinting technology may find widespread applications in regenerative medicine, drug screening, and potentially disease modeling.
Journal ArticleDOI
Instrumented cardiac microphysiological devices via multimaterial three-dimensional printing
Johan Ulrik Lind,Johan Ulrik Lind,Travis Alexander Busbee,Travis Alexander Busbee,Alexander D. Valentine,Alexander D. Valentine,Francesco S. Pasqualini,Francesco S. Pasqualini,Hongyan Yuan,Hongyan Yuan,Hongyan Yuan,Moran Yadid,Moran Yadid,Sung-Jin Park,Sung-Jin Park,Arda Kotikian,Arda Kotikian,Alexander P. Nesmith,Alexander P. Nesmith,Patrick H. Campbell,Patrick H. Campbell,Joost J. Vlassak,Jennifer A. Lewis,Jennifer A. Lewis,Kevin Kit Parker,Kevin Kit Parker +25 more
TL;DR: Six functional inks are designed, based on piezo-resistive, high conductance, and biocompatible soft materials that enable integration of soft strain gauge sensors within micro-architectures that guide the self-assembly of physio-mimetic laminar cardiac tissues via multi-material 3D printing.
References
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Journal ArticleDOI
Human embryonic stem cells can differentiate into myocytes with structural and functional properties of cardiomyocytes.
Izhak Kehat,Dorit Kenyagin-Karsenti,Mirit Snir,Hana Segev,Michal Amit,Amira Gepstein,Erella Livne,Ofer Binah,Joseph Itskovitz-Eldor,Lior Gepstein +9 more
TL;DR: The human ES cell--derived cardiomyocytes displayed structural and functional properties of early-stage cardiomers, which may have significant impact on the study of early human cardiac differentiation, functional genomics, pharmacological testing, cell therapy, and tissue engineering.
Journal ArticleDOI
Cardiac Hypertrophy: The Good, the Bad, and the Ugly
TL;DR: Recent insights into hypertrophic signaling are summarized, several novel antihypertrophic strategies are considered and modulation of myocardial growth without adversely affecting contractile function is increasingly recognized as a potentially auspicious approach in the prevention and treatment of heart failure.
Journal ArticleDOI
Human cardiovascular progenitor cells develop from a KDR + embryonic-stem-cell-derived population
Lei Yang,Mark H. Soonpaa,Eric Adler,Torsten K. Roepke,Steven J. Kattman,Marion Kennedy,Els Henckaerts,Kristina Bonham,Geoffrey W. Abbott,R. Michael Linden,R. Michael Linden,Loren J. Field,Gordon Keller,Gordon Keller +13 more
TL;DR: Analysis of the development of the cardiovascular lineages in human embryonic stem cell differentiation cultures identifies a human cardiovascular progenitor that defines one of the earliest stages of human cardiac development.
Journal ArticleDOI
Functional Cardiomyocytes Derived From Human Induced Pluripotent Stem Cells
Jianhua Zhang,Gisela F. Wilson,Andrew G. Soerens,Chad H. Koonce,Junying Yu,Sean P. Palecek,James A. Thomson,Timothy J. Kamp +7 more
TL;DR: It is concluded that human iPS cells are a viable option as an autologous cell source for cardiac repair and a powerful tool for cardiovascular research.
PatentDOI
Differentiation of human embryonic stem cells to cardiomyocytes
TL;DR: This is the first demonstration of induction ofcardiomyocyte differentiation in hES cells that do not undergo spontaneous cardiogenesis and provides a model for the study of human cardiomyocytes in culture and could be a step forward in the development of cardiomeocyte transplantation therapies.