Co-option of Sox3 as the male-determining factor on the Y chromosome in the fish Oryzias dancena
Yusuke Takehana,Yusuke Takehana,Masaru Matsuda,Taijun Myosho,Maximiliano L. Suster,Koichi Kawakami,Koichi Kawakami,Tadasu Shin-I,Yuji Kohara,Yoko Kuroki,Atsushi Toyoda,Atsushi Toyoda,Asao Fujiyama,Asao Fujiyama,Asao Fujiyama,Satoshi Hamaguchi,Mitsuru Sakaizumi,Kiyoshi Naruse,Kiyoshi Naruse +18 more
TLDR
This work uses positional cloning to identify the sex-determining locus of a medaka-related fish, Oryzias dancena, and finds that the locus on the Y chromosome contains a cis-regulatory element that upregulates neighbouring Sox3 expression in developing gonad.Abstract:
Sex chromosomes harbour a primary sex-determining signal that triggers sexual development of the organism. However, diverse sex chromosome systems have been evolved in vertebrates. Here we use positional cloning to identify the sex-determining locus of a medaka-related fish, Oryzias dancena, and find that the locus on the Y chromosome contains a cis-regulatory element that upregulates neighbouring Sox3 expression in developing gonad. Sex-reversed phenotypes in Sox3Y transgenic fish, and Sox3Y loss-of-function mutants all point to its critical role in sex determination. Furthermore, we demonstrate that Sox3 initiates testicular differentiation by upregulating expression of downstream Gsdf, which is highly conserved in fish sex differentiation pathways. Our results not only provide strong evidence for the independent recruitment of Sox3 to male determination in distantly related vertebrates, but also provide direct evidence that a novel sex determination pathway has evolved through co-option of a transcriptional regulator potentially interacted with a conserved downstream component. Sex chromosomes harbour specific sequences that determine the sexual development of the organism; yet these sequences remain unknown for many species. Here, Takehana et al. show that, similarly to mammals, Sox3 on the Y chromosome is the male-determining factor in the medaka-related fish Oryzias dancena.read more
Citations
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Vertebrate sex determination: evolutionary plasticity of a fundamental switch.
TL;DR: The unusual plasticity in the bipotential system of sex determination and some of the diverse mechanisms that have evolved to control this critical developmental decision are considered, including strong genetic pathways, environmental influences and epigenetic regulation.
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Genetic basis and biotechnological manipulation of sexual dimorphism and sex determination in fish
TL;DR: This review summarizes the progress in the mechanisms of fish sex determination and identification of sex-determining genes to provide some directive and objective thinking for further research in this field.
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A Tandem Duplicate of Anti-Müllerian Hormone with a Missense SNP on the Y Chromosome Is Essential for Male Sex Determination in Nile Tilapia, Oreochromis niloticus.
Minghui Li,Yun-Lv Sun,Jiue Zhao,Hongjuan Shi,Sheng Zeng,Kai Ye,Dongneng Jiang,Linyan Zhou,Lina Sun,Wenjing Tao,Yoshitaka Nagahama,Thomas D. Kocher,Deshou Wang +12 more
TL;DR: It is shown that amhy, a Y-specific duplicate of the anti-Müllerian hormone (amh) gene, induces male sex determination in Nile tilapia and the conserved roles of TGF-β signaling pathway in fish sex determination are highlighted.
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Wild Sex in Zebrafish: Loss of the Natural Sex Determinant in Domesticated Strains
Catherine Wilson,Samantha K. High,Braedan M. McCluskey,Angel Amores,Yi-Lin Yan,Tom A. Titus,Jennifer L. Anderson,Peter Batzel,Michael J. Carvan,Manfred Schartl,John H. Postlethwait +10 more
TL;DR: Results suggest that zebrafish in nature possess a WZ/ZZ sex-determination mechanism with a major determinant lying near the right telomere of chromosome 4 that was modified during domestication.
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Plasticity of gene-regulatory networks controlling sex determination: of masters, slaves, usual suspects, newcomers, and usurpators
Amaury Herpin,Manfred Schartl +1 more
TL;DR: Recently, sex‐determining genes from several animal species have been identified and new studies have elucidated some novel regulatory interactions and biological functions of the downstream network, particularly in vertebrates, which considerably changed classical perception of a simple linear developmental cascade that makes the decision for the embryo to develop as male or female.
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