Comprehensive annotation of the transcriptome of the human fungal pathogen Candida albicans using RNA-seq
Vincent M. Bruno,Zhong Wang,Sadie L. Marjani,Ghia Euskirchen,Jeffrey Martin,Gavin Sherlock,Michael Snyder +6 more
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TLDR
This comprehensive transcriptome analysis significantly enhances the current genome annotation of C. albicans, a necessary framework for a complete understanding of the molecular mechanisms of pathogenesis for this important eukaryotic pathogen.Abstract:
Candida albicans is the major invasive fungal pathogen of humans, causing diseases ranging from superficial mucosal infections to disseminated, systemic infections that are often lifethreatening. We have used massively parallel high-throughput sequencing of cDNA (RNA-seq) to generate a high-resolution map of the C. albicans transcriptome under several different environmental conditions. We have quantitatively determined all of the regions that are transcribed under these different conditions, and have identified 602 novel transcriptionally active regions (TARs) and numerous novel introns that are not represented in the current genome annotation. Interestingly, the expression of many of these TARs is regulated in a condition-specific manner. This comprehensive transcriptome analysis significantly enhances the current genome annotation of C. albicans, a necessary framework for a complete understanding of the molecular mechanisms of pathogenesis for this important eukaryotic pathogen.read more
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Artemis: an integrated platform for visualization and analysis of high-throughput sequence-based experimental data
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Dual RNA-seq of pathogen and host
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A Recently Evolved Transcriptional Network Controls Biofilm Development in Candida albicans
Clarissa J. Nobile,Emily P. Fox,Jeniel E. Nett,Trevor R. Sorrells,Quinn M. Mitrovich,Aaron D. Hernday,Brian B. Tuch,David R. Andes,Alexander D. Johnson +8 more
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A systematically improved high quality genome and transcriptome of the human blood fluke Schistosoma mansoni.
Anna V. Protasio,Isheng J. Tsai,A K Babbage,Sarah Nichol,Martin Hunt,Martin Aslett,Nishadi De Silva,Giles S. Velarde,Tim J. Anderson,Richard Clark,Claire Davidson,Gary P. Dillon,Nancy Holroyd,Philip T. LoVerde,Christine Lloyd,Jacquelline McQuillan,Guilherme Oliveira,Thomas D. Otto,Sophia J. Parker-Manuel,Michael A. Quail,R. Alan Wilson,Adhemar Zerlotini,David W. Dunne,Matthew Berriman +23 more
TL;DR: The upgraded genome of Schistosoma mansoni will form a fundamental dataset to underpin further advances in schistosome research and is consolidated into a searchable format within the GeneDB and SchistoDB databases.
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Genome sequence of the insect pathogenic fungus Cordyceps militaris , a valued traditional chinese medicine
Peng Zheng,Yongliang Xia,Guohua Xiao,Chenghui Xiong,Xiao Hu,Siwei Zhang,Huajun Zheng,Yin Huang,Yan Zhou,Shengyue Wang,Guoping Zhao,Guoping Zhao,Xingzhong Liu,Raymond J. St. Leger,Chengshu Wang +14 more
TL;DR: It is established that C. militaris is sexually heterothallic but, very unusually, fruiting can occur without an opposite mating-type partner, which suggests a more restricted ecology.
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TL;DR: An approach that generates several billion bases of accurate nucleotide sequence per experiment at low cost is reported, effective for accurate, rapid and economical whole-genome re-sequencing and many other biomedical applications.
Journal ArticleDOI
Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent.
TL;DR: O(2) (-) is made by leukocytes under circumstances which suggest that it may be involved in bacterial killing, and is identified as the agent responsible for the leukocyte-mediated reduction of cytochrome c.
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