Journal ArticleDOI
Delayed flowering time in Arabidopsis and Brassica rapa by the overexpression of FLOWERING LOCUS C (FLC) homologs isolated from Chinese Cabbage (Brassica rapa L. ssp. pekinensis)
Kim Soo Yun,Beom Seok Park,Soo Jin Kwon,Jung Sun Kim,Myung-Ho Lim,Young-Doo Park,Dool Yi Kim,Seok Chul Suh,Yong Moon Jin,Ji Hoon Ahn,Yeon-Hee Lee +10 more
TLDR
The results suggest that the BrFLC genes act similarly to AtFLC, a technique for controlling flowering time in Chinese cabbage and other crops to produce high yields of vegetative tissues.Abstract:
Chinese cabbage plants remain in the vegetative growth phase until they have experienced prolonged exposure to cold temperature, known as vernalization. This inhibition of flowering is caused by the high levels of FLOWERING LOCUS C (FLC) expression. To increase the product value of Chinese cabbage by inhibiting the floral transition, three genes (BrFLC1, BrFLC2, and BrFLC3) homologous to the AtFLC gene, which encodes a floral repressor, were isolated from the Chinese cabbage ‘Chiifu’. These genes showed high similarity to AtFLC, although the putative BrFLC1 protein contained ten more residues than AtFLC. The BrFLC genes were expressed ubiquitously, except that BrFLC3 was not expressed in roots. BrFLC1 and BrFLC2 showed stronger expression than BrFLC3 in unvernalized and vernalized Chinese cabbage. The expression levels of the three BrFLC genes were lower in an early-flowering Chinese cabbage, suggesting that the BrFLC transcript level was associated with flowering time. Constitutive expression of the BrFLC genes in Arabidopsis significantly delayed flowering, which was also observed in transgenic Chinese cabbage overexpressing BrFLC3. These results suggest that the BrFLC genes act similarly to AtFLC. Our results provide a technique for controlling flowering time in Chinese cabbage and other crops to produce high yields of vegetative tissues.read more
Citations
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Toward a synthetic understanding of the role of phenology in ecology and evolution
TL;DR: The relationship betweenphenology and life history, the distinction between organismal- and population-level perspectives on phenology and the influence of phenology on evolutionary processes, communities and ecosystems are discussed.
Journal ArticleDOI
Flowering time control and applications in plant breeding
Christian Jung,Andreas E. Müller +1 more
TL;DR: The major floral regulatory pathways are reviewed and current and novel strategies for altering bolting and flowering behavior in crop plants are discussed.
Journal ArticleDOI
The pangenome of an agronomically important crop plant Brassica oleracea
Agnieszka A. Golicz,Philipp E. Bayer,Guy C. Barker,Patrick P. Edger,Hyeran Kim,Paula Martinez,Chon-Kit Kenneth Chan,Anita A. Severn-Ellis,W. Richard McCombie,Isobel A. P. Parkin,Andrew H. Paterson,J. Chris Pires,Andrew G. Sharpe,Haibao Tang,Graham R. Teakle,Christopher D. Town,Jacqueline Batley,David Edwards +17 more
TL;DR: Several genes displaying presence/absence variation are annotated with functions related to major agronomic traits, including disease resistance, flowering time, glucosinolate metabolism and vitamin biosynthesis.
Journal ArticleDOI
Genetic and physiological bases for phenological responses to current and predicted climates
Amity M. Wilczek,Liana T. Burghardt,Alexander R. Cobb,Martha D. Cooper,Stephen Welch,Johanna Schmitt +5 more
TL;DR: It is shown that the expression of phenological traits including flowering depends critically on the growth season, and an integrated life-history approach to phenology is outlined in which the timing of later life- history events can be contingent on the environmental cues regulating earlier life stages.
Journal ArticleDOI
A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time
TL;DR: Findings suggest that a naturally occurring splicing mutation in the BrFLC1 gene contributes greatly to flowering-time variation in B. rapa.
References
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Journal ArticleDOI
Genomic sequencing
George M. Church,Walter Gilbert +1 more
TL;DR: The genomic sequencing procedures are applicable to the analysis of genetic polymorphisms, DNA methylation at deoxycytidines, and nucleic acid-protein interactions at single nucleotide resolution.
Journal ArticleDOI
FLOWERING LOCUS C Encodes a Novel MADS Domain Protein That Acts as a Repressor of Flowering
TL;DR: This study reports that flc null mutations result in early flowering, demonstrating that the role of active FLC alleles is to repress flowering, and proposes that the level of FLC activity acts through a rheostat-like mechanism to control flowering time in Arabidopsis and that modulation of F LC expression is a component of the vernalization response.
Journal ArticleDOI
A small-scale procedure for the rapid isolation of plant RNAs
Journal ArticleDOI
Molecular Analysis of FRIGIDA, a Major Determinant of Natural Variation in Arabidopsis Flowering Time
TL;DR: This work cloned FRI and analyzed the molecular basis of the allelic variation at the FRIGIDA locus, finding that loss-of-function mutations at FRI have provided the basis for the evolution of many early-flowering ecotypes.
Journal ArticleDOI
The FLF MADS Box Gene: A Repressor of Flowering in Arabidopsis Regulated by Vernalization and Methylation
Candice C. Sheldon,Joanne Elizabeth Burn,Pascual Perez,James D. Metzger,Jennifer A. Edwards,Jennifer A. Edwards,W. James Peacock,Elizabeth S. Dennis +7 more
TL;DR: The flf-1 mutant requires a greater than normal amount of an exogenous gibberellin to decrease flowering time compared with the wild type or with vernalization-responsive late-flowering mutants, suggesting that the FLF gene product may block the promotion of flowering by GAs.
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