Diverse molecular functions of Hu proteins.
Melissa N. Hinman,Hua Lou +1 more
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TLDR
This communication will focus on these molecular events and review the current understanding of how Hu proteins mediate them, and emphasis will be put on the nuclear functions of these proteins, which were recently discovered.Abstract:
Hu proteins are RNA-binding proteins involved in diverse biological processes. The neuronal members of the Hu family, HuB, HuC, and HuD play important roles in neuronal differentiation and plasticity, while the ubiquitously expressed family member, HuR, has numerous functions mostly related to cellular stress response. The pivotal roles of Hu proteins are dictated by their molecular functions affecting a large number of target genes. Hu proteins affect many post-transcriptional aspects of RNA metabolism, from splicing to translation. In this communication, we will focus on these molecular events and review our current understanding of how Hu proteins mediate them. In particular, emphasis will be put on the nuclear functions of these proteins, which were recently discovered. Three examples including calcitonin/calcitonin gene-related peptide, neurofibromatosis type 1, and Ikaros will be discussed in detail. In addition, an intriguing theme of antagonism between Hu proteins and other AU-rich sequence binding proteins will be discussed.read more
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Regulation of mRNA Translation and Stability by microRNAs
TL;DR: In this article, the authors describe principles of miRNA-mRNA interactions and proteins that interact with miRNAs and function in miRNA mediated repression, and discuss the multiple, often contradictory, mechanisms that miRNA have been reported to use, which cause translational repression and mRNA decay.
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LincRNA-p21 Suppresses Target mRNA Translation
Je Hyun Yoon,Kotb Abdelmohsen,Subramanya Srikantan,Xiaoling Yang,Jennifer L. Martindale,Supriyo De,Maite Huarte,Ming Zhan,Kevin G. Becker,Myriam Gorospe +9 more
TL;DR: It is proposed that HuR controls translation of a subset of target mRNAs by influencing lincRNA-p21 levels, which in turn derepressed JunB and β-catenin translation and increased the levels of these proteins.
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Alternative pre-mRNA splicing regulation in cancer: pathways and programs unhinged
Charles J. David,James L. Manley +1 more
TL;DR: Emerging insights into this process indicate that pathways that are frequently deregulated in cancer often play important roles in promoting aberrant splicing, which in turn contributes to all aspects of tumor biology.
Journal ArticleDOI
Transcriptome-wide analysis of regulatory interactions of the RNA-binding protein HuR.
Svetlana Lebedeva,Marvin Jens,Kathrin Theil,Björn Schwanhäusser,Matthias Selbach,Markus Landthaler,Nikolaus Rajewsky +6 more
TL;DR: Thousands of direct and functional HuR targets are identified, a human miRNA controlled by HuR is found, and a role for HuR in splicing is proposed, validating functionality.
Journal ArticleDOI
Integrative Regulatory Mapping Indicates that the RNA-Binding Protein HuR Couples Pre-mRNA Processing and mRNA Stability
Neelanjan Mukherjee,David L. Corcoran,Jeffrey Nusbaum,Jeffrey Nusbaum,David W. Reid,Stoyan Georgiev,Markus Hafner,Markus Hafner,Manuel Ascano,Manuel Ascano,Thomas Tuschl,Thomas Tuschl,Uwe Ohler,Jack D. Keene +13 more
TL;DR: Comparison of the spatial patterns surrounding HuR and miRNA binding sites provided functional evidence for HuR-dependent antagonism of proximal miRNA-mediated repression and it is concluded that HuR coordinates gene expression outcomes at multiple interconnected steps of RNA processing.
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