Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imaging
Hideyuki Yaginuma,Shinnosuke Kawai,Kazuhito V. Tabata,Keisuke Tomiyama,Akira Kakizuka,Tamiki Komatsuzaki,Hiroyuki Noji,Hiromi Imamura +7 more
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TLDR
A new genetically-encoded ratiometric fluorescent ATP indicator “QUEEN”, which is composed of a single circularly-permuted fluorescent protein and a bacterial ATP binding protein, is developed that, unlike previous FRET-based indicators, was apparently insensitive to bacteria growth rate changes.Abstract:
Diversity in ATP concentrations in a single bacterial cell population revealed by quantitative single-cell imagingread more
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Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.
TL;DR: In an effort to encapsulate the breadth over which fluorescent biosensors have expanded, this work endeavored to assemble a comprehensive list of published engineered bios Sensors, and discusses many of the molecular designs utilized in their development.
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A genetically encoded single-wavelength sensor for imaging cytosolic and cell surface ATP.
Mark A. Lobas,Rongkun Tao,Jun Nagai,Mira T. Kronschläger,Mira T. Kronschläger,Philip M. Borden,Jonathan S. Marvin,Loren L. Looger,Baljit S. Khakh +8 more
TL;DR: A new ATP sensor that can be targeted to the membrane or cytosol is developed, and represents promising reagents for imaging ATP in the extracellular space and within cells during a variety of settings, and for further application-specific refinements.
Journal ArticleDOI
ATP-Dependent Dynamic Protein Aggregation Regulates Bacterial Dormancy Depth Critical for Antibiotic Tolerance.
Yingying Pu,Yingxing Li,Xin Jin,Tian Tian,Qi Ma,Ziyi Zhao,Ssu-yuan Lin,Zhanghua Chen,Binghui Li,Gung Yao,Mark C. Leake,Chien-Jung Lo,Fan Bai +12 more
TL;DR: Monitoring of bacterial antibiotic tolerance and regrowth at the single-cell level found that each individual survival cell shows different "dormancy depth," which in return regulates the lag time for cell resuscitation after removal of antibiotic.
Journal ArticleDOI
Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.
Bjarne Hove-Jensen,Kasper R. Andersen,Mogens Kilstrup,Jan Martinussen,Robert L. Switzer,Martin Willemoës +5 more
TL;DR: The results of these analyses are unified with recent progress in molecular enzymology and the elucidation of the three-dimensional structures of PRPP synthases from eubacteria, archaea, and humans.
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Guidelines on experimental methods to assess mitochondrial dysfunction in cellular models of neurodegenerative diseases
Niamh M. C. Connolly,Pierre Theurey,Vera Adam-Vizi,Nicolas G. Bazan,Paolo Bernardi,Paolo Bernardi,Juan P. Bolaños,Carsten Culmsee,Valina L. Dawson,Mohanish Deshmukh,Michael R. Duchen,Heiko Düssmann,Gary Fiskum,Maria F. Galindo,Giles E. Hardingham,J. Marie Hardwick,Mika B. Jekabsons,Elizabeth A. Jonas,Joaquín Jordán,Stuart A. Lipton,Stuart A. Lipton,Giovanni Manfredi,Mark P. Mattson,BethAnn McLaughlin,Axel Methner,Anne N. Murphy,Michael P. Murphy,David G. Nicholls,Brian M. Polster,Tullio Pozzan,Tullio Pozzan,Rosario Rizzuto,Rosario Rizzuto,Jorgina Satrústegui,Ruth S. Slack,Raymond A. Swanson,Russell H. Swerdlow,Yvonne Will,Zheng Ying,Alvin Joselin,Anna Gioran,Catarina Moreira Pinho,Orla Watters,Manuela Salvucci,Irene Llorente-Folch,David S. Park,Daniele Bano,Maria Ankarcrona,Paola Pizzo,Paola Pizzo,Jochen H. M. Prehn +50 more
TL;DR: Details on and discuss pitfalls of existing experimental approaches to assess mitochondrial function in in vitro cellular models of neurodegenerative diseases, including specific protocols for the measurement of oxygen consumption rate in primary neuron cultures, and single-neuron, time-lapse fluorescence imaging of the mitochondrial membrane potential and mitochondrial NAD(P)H.
References
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Avigdor Eldar,Michael B. Elowitz +1 more
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