Efficacy and breadth of adjuvanted SARS-CoV-2 receptor-binding domain nanoparticle vaccine in macaques.
Hannah A.D. King,Hannah A.D. King,M. Gordon Joyce,M. Gordon Joyce,Ines Lakhal-Naouar,Ines Lakhal-Naouar,Aslaa Ahmed,Camila Macedo Cincotta,Camila Macedo Cincotta,Caroline Subra,Caroline Subra,Kristina K. Peachman,Holly R. Hack,Holly R. Hack,Rita E. Chen,Paul V. Thomas,Paul V. Thomas,Wei-Hung Chen,Wei-Hung Chen,Rajeshwer S. Sankhala,Rajeshwer S. Sankhala,Agnes Hajduczki,Agnes Hajduczki,Elizabeth J. Martinez,Elizabeth J. Martinez,Caroline E. Peterson,Caroline E. Peterson,William C. Chang,William C. Chang,Misook Choe,Misook Choe,Clayton A. Smith,Jarrett A. Headley,Jarrett A. Headley,Hanne A. Elyard,Anthony L. Cook,Alexander R. A. Anderson,Alexander R. A. Anderson,Kathryn McGuckin Wuertz,Ming Dong,Ming Dong,Isabella Swafford,Isabella Swafford,James Brett Case,Jeffrey R. Currier,Kerri G. Lal,Kerri G. Lal,Mihret F. Amare,Mihret F. Amare,Vincent Dussupt,Vincent Dussupt,Sebastian Molnar,Sebastian Molnar,Sharon P. Daye,Xiankun Zeng,Erica K. Barkei,Kendra J. Alfson,Hilary M. Staples,Ricardo Carrion,Shelly J. Krebs,Shelly J. Krebs,Dominic Paquin-Proulx,Dominic Paquin-Proulx,Nicos Karasavvas,Nicos Karasavvas,Victoria R. Polonis,Linda L. Jagodzinski,Sandhya Vasan,Sandhya Vasan,Paul T. Scott,Yaoxing Huang,Manoj S. Nair,David D. Ho,Natalia de Val,Michael S. Diamond,Mark G. Lewis,Mangala Rao,Gary R. Matyas,Gregory D. Gromowski,Sheila A. Peel,Nelson L. Michael,Kayvon Modjarrad,Diane L. Bolton,Diane L. Bolton +83 more
TLDR
In this article, a ferritin nanoparticle vaccine displaying the receptor-binding domain of the SARS-CoV-2 spike protein (RFN) adjuvanted with Army Liposomal Formulation QS-21 (ALFQ) was evaluated.Abstract:
Emergence of novel variants of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) underscores the need for next-generation vaccines able to elicit broad and durable immunity. Here we report the evaluation of a ferritin nanoparticle vaccine displaying the receptor-binding domain of the SARS-CoV-2 spike protein (RFN) adjuvanted with Army Liposomal Formulation QS-21 (ALFQ). RFN vaccination of macaques using a two-dose regimen resulted in robust, predominantly Th1 CD4+ T cell responses and reciprocal peak mean serum neutralizing antibody titers of 14,000 to 21,000. Rapid control of viral replication was achieved in the upper and lower airways of animals after high-dose SARS-CoV-2 respiratory challenge, with undetectable replication within 4 d in seven of eight animals receiving 50 µg of RFN. Cross-neutralization activity against SARS-CoV-2 variant B.1.351 decreased only approximately twofold relative to WA1/2020. In addition, neutralizing, effector antibody and cellular responses targeted the heterotypic SARS-CoV-1, highlighting the broad immunogenicity of RFN-ALFQ for SARS-CoV-like Sarbecovirus vaccine development.read more
Citations
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Scientific rationale for developing potent RBD-based vaccines targeting COVID-19.
Harry Kleanthous,Judith M. Silverman,Karen W. Makar,In-Kyu Yoon,Nicholas Jackson,D. W. Vaughn +5 more
TL;DR: In this paper, the authors reviewed the data supporting the non-inferiority of RBD as a vaccine immunogen compared to full-length S-protein vaccines with respect to humoral and cellular immune responses against both the prototype pandemic SARS-CoV-2 isolate and emerging variants of concern.
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Mutations of SARS-CoV-2 spike protein: Implications on immune evasion and vaccine-induced immunity.
Hylemariam Mihiretie Mengist,Arnaud John Kombe Kombe,Daniel Mekonnen,Abtie Abebaw,Melese Getachew,Tengchuan Jin +5 more
TL;DR: In this article, the authors discuss the implications of SARS-CoV-2 spike protein mutations on immune evasion and vaccine-induced immunity and forward directions which could contribute to future studies focusing on designing effective vaccines and/or immunotherapies to consider viral evolution.
Journal ArticleDOI
A SARS-CoV-2 spike ferritin nanoparticle vaccine protects hamsters against Alpha and Beta virus variant challenge.
Kathryn McGuckin Wuertz,Erica K. Barkei,Wei-Hung Chen,Wei-Hung Chen,Elizabeth J. Martinez,Elizabeth J. Martinez,Ines Lakhal-Naouar,Ines Lakhal-Naouar,Linda L. Jagodzinski,Dominic Paquin-Proulx,Dominic Paquin-Proulx,Gregory D. Gromowski,Isabella Swafford,Isabella Swafford,Akshaya Ganesh,Akshaya Ganesh,Ming Dong,Ming Dong,Xiankun Zeng,Paul V. Thomas,Paul V. Thomas,Rajeshwer S. Sankhala,Rajeshwer S. Sankhala,Agnes Hajduczki,Agnes Hajduczki,Caroline E. Peterson,Caroline E. Peterson,Caitlin Kuklis,Sandrine Soman,Lindsay Wieczorek,Lindsay Wieczorek,Michelle Zemil,Michelle Zemil,Alexander R. A. Anderson,Alexander R. A. Anderson,Janice Darden,Janice Darden,Heather Hernandez,Heather Hernandez,Hannah Grove,Hannah Grove,Vincent Dussupt,Vincent Dussupt,Holly R. Hack,Holly R. Hack,Rafael De La Barrera,Stasya Zarling,James F. Wood,Jeffrey W. Froude,Matthew Gagne,Amy R. Henry,Elham Bayat Mokhtari,Prakriti Mudvari,Shelly J. Krebs,Shelly J. Krebs,Andrew Pekosz,Jeffrey R. Currier,Swagata Kar,Maciel Porto,Adrienne Winn,Kamil Radzyminski,Mark G. Lewis,Sandhya Vasan,Mehul S. Suthar,Victoria R. Polonis,Gary R. Matyas,Eli Boritz,Daniel C. Douek,Robert A. Seder,Sharon P. Daye,Mangala Rao,Sheila A. Peel,M. Gordon Joyce,M. Gordon Joyce,Diane L. Bolton,Diane L. Bolton,Nelson L. Michael,Kayvon Modjarrad +77 more
TL;DR: In this paper, the authors evaluated whether a SARS-CoV-2 spike ferritin nanoparticle vaccine (SpFN), adjuvanted with the Army Liposomal Formulation QS21 (ALFQ), conferred protection against the Alpha (B.1.7), and Beta (B1.351) VOCs in Syrian golden hamsters.
Journal ArticleDOI
SARS-CoV-2 S2–targeted vaccination elicits broadly neutralizing antibodies
TL;DR: The authors showed that prior HCoVOC43 S-targeted immunity primes neutralizing antibody responses to otherwise subimmunogenic SARS-CoV-2 S exposure and promotes S2-targeting antibody responses.
Journal ArticleDOI
SARS-CoV-2 spike conformation determines plasma neutralizing activity elicited by a wide panel of human vaccines
John E. Bowen,Young-Jun Park,Cameron Stewart,Jack T Brown,William K Sharkey,Alexandra C. Walls,Anshu Joshi,Kaitlin R. Sprouse,Matthew McCallum,M. Alejandra Tortorici,Nicholas Franko,Jennifer Logue,Ignacio Mazzitelli,Annalee W. Nguyen,Rui P. Silva,Yimin Huang,Jun Siong Low,Josipa Jerak,Sasha W Tiles,Kumail Ahmed,Asefa Shariq,Jennifer M. Dan,Zeli Zhang,Daniela Weiskopf,Alessandro Sette,Gyorgy Snell,Christine M. Posavad,Najeeha Talat Iqbal,Jorge Geffner,Alessandra Bandera,Andrea Gori,Federica Sallusto,Jennifer A. Maynard,Shane Crotty,Wesley C. Van Voorhis,Carlos Simmerling,Renata Grifantini,Helen Y. Chu,Davide Corti,David Veesler +39 more
TL;DR: The authors showed that vaccines containing prefusion-stabilizing S mutations elicit antibody responses in humans with enhanced recognition of S and the S1 subunit relative to postfusion S, as compared to vaccines lacking these mutations or natural infection.
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