Enzyme immobilisation in biocatalysis : Why, what and how
Roger A. Sheldon,Sander van Pelt +1 more
TLDR
An overview of the why, what and how of enzyme immobilisation for use in biocatalysis is presented and emphasis is placed on relatively recent developments, such as the use of novel supports such as mesoporous silicas, hydrogels, and smart polymers, and cross-linked enzyme aggregates (CLEAs).Abstract:
In this tutorial review, an overview of the why, what and how of enzyme immobilisation for use in biocatalysis is presented. The importance of biocatalysis in the context of green and sustainable chemicals manufacture is discussed and the necessity for immobilisation of enzymes as a key enabling technology for practical and commercial viability is emphasised. The underlying reasons for immobilisation are the need to improve the stability and recyclability of the biocatalyst compared to the free enzyme. The lower risk of product contamination with enzyme residues and low or no allergenicity are further advantages of immobilised enzymes. Methods for immobilisation are divided into three categories: adsorption on a carrier (support), encapsulation in a carrier, and cross-linking (carrier-free). General considerations regarding immobilisation, regardless of the method used, are immobilisation yield, immobilisation efficiency, activity recovery, enzyme loading (wt% in the biocatalyst) and the physical properties, e.g. particle size and density, hydrophobicity and mechanical robustness of the immobilisate, i.e. the immobilised enzyme as a whole (enzyme + support). The choice of immobilisate is also strongly dependent on the reactor configuration used, e.g. stirred tank, fixed bed, fluidised bed, and the mode of downstream processing. Emphasis is placed on relatively recent developments, such as the use of novel supports such as mesoporous silicas, hydrogels, and smart polymers, and cross-linked enzyme aggregates (CLEAs).read more
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Taguchi design-assisted co-immobilization of lipase A and B from Candida antarctica onto chitosan: Characterization, kinetic resolution application, and docking studies
Katerine da Silva Moreira,André L. B. de Oliveira,Lourembergue Saraiva de Moura Junior,Isamayra Germano de Sousa,Antônio Luthierre Gama Cavalcante,Francisco Simao Neto,Roberta Bussons Rodrigues Valério,Anderson Valério Chaves,Thiago de Sousa Fonseca,Thiago de Sousa Fonseca,Daniel Morais Vieira Cruz,Daniel Morais Vieira Cruz,Gledson Vieira Lima,Gledson Vieira Lima,Gabriel Paixão de Oliveira,Maria Cristiane Martins de Souza,Pierre Basílio Almeida Fechine,Marcos Carlos de Mattos,Aluísio Marques da Fonseca,José C. S. dos Santos,José C. S. dos Santos +20 more
TL;DR: In this paper, the simultaneous co-immobilization by covalent binding of lipase A from Candida antarctica (CALA) and lipase B from CALB in glutaraldehyde activated chitosan (CHI) was optimized using the Taguchi method.
Journal ArticleDOI
Different strategies for the lipase immobilization on the chitosan based supports and their applications.
Fatemeh Rafiee,M. Rezaee +1 more
TL;DR: In this paper, the authors highlight several aspects of lipase immobilization on the surface of chitosan support, including physical adsorption to covalent bonding and cross-linking with their benefits and drawbacks.
Journal ArticleDOI
Taguchi design-assisted co-immobilization of lipase A and B from Candida antarctica onto chitosan: Characterization, kinetic resolution application, and docking studies
TL;DR: In this paper , the simultaneous co-immobilization by covalent binding of lipase A from Candida antarctica (CALA) and lipase B from CALB in glutaraldehyde activated chitosan (CHI) was optimized using the Taguchi method.
Journal ArticleDOI
Reuse of anion exchangers as supports for enzyme immobilization: Reinforcement of the enzyme-support multiinteraction after enzyme inactivation
Jose J. Virgen-Ortíz,Sara Peirce,Sara Peirce,Veymar G. Tacias-Pascacio,Vicente Cortes-Corberan,Antonio Marzocchella,Maria Russo,Roberto Fernandez-Lafuente +7 more
TL;DR: Recovery of a support fully free of protein molecules is not an easy objective after enzyme inactivation, because the inactivated enzymes seemed to unfold increasing in a great way the interaction with the support, driving to a very strong enzyme-support multi-interaction that difficulty its desorption.
Journal ArticleDOI
Utilization of two modified layered doubled hydroxides as supports for immobilization of Candida rugosa lipase.
TL;DR: Kinetic parameters obtained from the Michaelis-Menten equation on MLDH1 and MLDH2 were comparable to free enzyme, and Kinetic parameters obtaining from theMichaelis- Mentsen equation for immobilization of Candida rugosa lipase on modified LDHs with monolayer surfactants were comparableto free enzyme.
References
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Engineering the third wave of biocatalysis
Uwe T. Bornscheuer,Gjalt W. Huisman,Romas J. Kazlauskas,Romas J. Kazlauskas,Stefan Lutz,Jeffrey C. Moore,Karen Robins +6 more
TL;DR: Applications of protein-engineered biocatalysts ranging from commodity chemicals to advanced pharmaceutical intermediates that use enzyme catalysis as a key step are discussed.
Journal ArticleDOI
Enzyme immobilization: The quest for optimum performance
TL;DR: Different methods for the immobilization of enzymes are critically reviewed, with emphasis on relatively recent developments, such as the use of novel supports, e.g., mesoporous silicas, hydrogels, and smart polymers, novel entrapment methods and cross-linked enzyme aggregates (CLEAs).
Journal ArticleDOI
Chemistry of Aerogels and Their Applications
Alain Pierre,Gerard Pajonk +1 more
TL;DR: Aerogels form a new class of solids showing sophisticated potentialities for a range of applications, and can develop very attractive physical and chemical properties not achievable by other means of low temperature soft chemical synthesis.
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Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance
TL;DR: The advantages and disadvantages of the different existing immobilization strategies to solve the different aforementioned enzyme limitations are given and some advice to select the optimal strategy for each particular enzyme and process is given.
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Application of chitin- and chitosan-based materials for enzyme immobilizations: a review
TL;DR: A review of the literature on enzymes immobilized on chitin- and chitosan-based materials, covering the last decade, is presented in this paper, where one hundred fifty-eight papers on 63 immobilized enzymes for multiplicity of applications ranging from wine, sugar and fish industry, through organic compounds removal from wastewaters to sophisticated biosensors for both in situ measurements of environmental pollutants and metabolite control in artificial organs, are reviewed.