Genome sequence of the palaeopolyploid soybean
Jeremy Schmutz,Steven B. Cannon,Jessica A. Schlueter,Jessica A. Schlueter,Jianxin Ma,Therese Mitros,William Nelson,David L. Hyten,Qijian Song,Qijian Song,Jay J. Thelen,Jianlin Cheng,Dong Xu,Uffe Hellsten,Gregory D. May,Yeisoo Yu,Tetsuya Sakurai,Taishi Umezawa,Madan K. Bhattacharyya,Devinder Sandhu,Babu Valliyodan,Erika Lindquist,Myron Peto,David Grant,Shengqiang Shu,David Goodstein,Kerrie Barry,Montona Futrell-Griggs,Brian Abernathy,Jianchang Du,Zhixi Tian,Liucun Zhu,Navdeep Gill,Trupti Joshi,Marc Libault,Ananad Sethuraman,Xue-Cheng Zhang,Kazuo Shinozaki,Henry T. Nguyen,Rod A. Wing,Perry B. Cregan,James E. Specht,Jane Grimwood,Daniel S. Rokhsar,Gary Stacey,Randy C. Shoemaker,Scott A. Jackson +46 more
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TLDR
An accurate soybean genome sequence will facilitate the identification of the genetic basis of many soybean traits, and accelerate the creation of improved soybean varieties.Abstract:
Soybean (Glycine max) is one of the most important crop plants for seed protein and oil content, and for its capacity to fix atmospheric nitrogen through symbioses with soil-borne microorganisms. We sequenced the 1.1-gigabase genome by a whole-genome shotgun approach and integrated it with physical and high-density genetic maps to create a chromosome-scale draft sequence assembly. We predict 46,430 protein-coding genes, 70% more than Arabidopsis and similar to the poplar genome which, like soybean, is an ancient polyploid (palaeopolyploid). About 78% of the predicted genes occur in chromosome ends, which comprise less than one-half of the genome but account for nearly all of the genetic recombination. Genome duplications occurred at approximately 59 and 13 million years ago, resulting in a highly duplicated genome with nearly 75% of the genes present in multiple copies. The two duplication events were followed by gene diversification and loss, and numerous chromosome rearrangements. An accurate soybean genome sequence will facilitate the identification of the genetic basis of many soybean traits, and accelerate the creation of improved soybean varieties.read more
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Identical Substitutions in Magnesium Chelatase Paralogs Result in Chlorophyll-Deficient Soybean Mutants
Benjamin W. Campbell,Dhananjay Mani,Shaun J. Curtin,Rebecca A. Slattery,Jean Michel Michno,Donald R. Ort,Donald R. Ort,Philip J. Schaus,Reid G. Palmer,James H. Orf,Robert M. Stupar +10 more
TL;DR: Protein sequence alignments of the two Mg-chelatase subunits indicated that the sites of amino acid modification in MinnGold, T219H, and CD-5 are highly conserved among photosynthetic species, suggesting that amino acid alterations in this critical domain may create competitive inhibitory interactions between the mutant and wild-type ChlI1a and Chli1b proteins.
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Integrated physical, genetic and genome map of chickpea ( Cicer arietinum L.)
Rajeev K. Varshney,Reyazul Rouf Mir,Sabhyata Bhatia,Mahendar Thudi,Yuqin Hu,Sarwar Azam,Yong Zhang,Deepa Jaganathan,Frank M. You,Jinliang Gao,Oscar Riera-Lizarazu,Oscar Riera-Lizarazu,Ming-Cheng Luo +12 more
TL;DR: Comprehensive analysis of markers in abiotic and biotic stress tolerance QTL regions led to identification of 654, 306 and 23 genes in drought tolerance “QTL-hotspot” region, Ascochyta blight resistanceQTL region and Fusarium wilt resistance QTL region, respectively.
Journal ArticleDOI
Major locus and other novel additive and epistatic loci involved in modulation of isoflavone concentration in soybean seeds
Juan J. Gutierrez-Gonzalez,Juan J. Gutierrez-Gonzalez,Tri D. Vuong,Rui Zhong,Oliver Yu,Jeong-Dong Lee,Grover Shannon,Mark R. Ellersieck,Henry T. Nguyen,David A. Sleper +9 more
TL;DR: The results suggest that a major locus, which alone accounted for up to 10% of the phenotypic variance for glycitein, and 35–37% for genistein, daidzein and the sum of all three soybean isoflavones, would be an excellent candidate to target for MAS.
Journal ArticleDOI
The characterization of soybean oil body integral oleosin isoforms and the effects of alkaline pH on them.
TL;DR: It was found that alkaline pH not only removed contaminated proteins but also oleosins, and more and more oleOSins were removed with increasing alkalin pH.
Journal ArticleDOI
Development and Validation of EST-SSR Markers from the Transcriptome of Adzuki Bean (Vigna angularis)
TL;DR: Large number of SSR-containing sequences and EST-SSR markers will be valuable for genetic analysis of the adzuki bean and related Vigna species through next-generation sequencing.
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