Global analysis of transcript and protein levels across the Plasmodium falciparum life cycle
Karine G. Le Roch,Jeffrey R. Johnson,Laurence Florens,Yingyao Zhou,Andrey Santrosyan,Munira Grainger,S. Frank Yan,Kim C. Williamson,Anthony A. Holder,Daniel J. Carucci,John R. Yates,Elizabeth A. Winzeler +11 more
TLDR
Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation.Abstract:
To investigate the role of post-transcriptional controls in the regulation of protein expression for the malaria parasite, Plasmodium falciparum, we have compared mRNA transcript and protein abundance levels for seven different stages of the parasite life cycle. A moderately high positive relationship between mRNA and protein abundance was observed for these stages; the most common discrepancy was a delay between mRNA and protein accumulation. Potentially post-transcriptionally regulated genes are identified, and families of functionally related genes were observed to share similar patterns of mRNA and protein accumulation.read more
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Journal ArticleDOI
On the Dependency of Cellular Protein Levels on mRNA Abundance.
TL;DR: It is concluded that transcript levels by themselves are not sufficient to predict protein levels in many scenarios and to thus explain genotype-phenotype relationships and that high-quality data quantifying different levels of gene expression are indispensable for the complete understanding of biological processes.
Journal ArticleDOI
Global signatures of protein and mRNA expression levels
TL;DR: This review summarizes the state of knowledge about large-scale measurements of absolute protein and mRNA expression levels, and the degree of correlation between the two parameters.
Journal ArticleDOI
A protein interaction network of the malaria parasite Plasmodium falciparum
Douglas J. LaCount,Marissa Vignali,Rakesh Chettier,Amit Phansalkar,Russell Bell,Jay R. Hesselberth,Lori W. Schoenfeld,Lori W. Schoenfeld,Irene Ota,Sudhir Sahasrabudhe,Cornelia Kurschner,Stanley Fields,Stanley Fields,Robert E. Hughes +13 more
TL;DR: From more than 32,000 yeast two-hybrid screens with P. falciparum protein fragments, 2,846 unique interactions are identified, most of which include at least one previously uncharacterized protein.
Journal ArticleDOI
Global survey of organ and organelle protein expression in mouse: combined proteomic and transcriptomic profiling.
Thomas Kislinger,Brian J. Cox,Anitha Kannan,Clement Chung,Pingzhao Hu,Alexandr Ignatchenko,Michelle S. Scott,Anthony O. Gramolini,Quaid Morris,Michael Hallett,Janet Rossant,Timothy P. Hughes,Brendan J. Frey,Andrew Emili +13 more
TL;DR: This molecular compendium, fully accessible via a searchable web-browser interface, serves as a reliable reference of the expressed tissue and organelle proteomes of a leading model mammal.
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The exopolysaccharide matrix modulates the interaction between 3D architecture and virulence of a mixed-species oral biofilm.
Jin Xiao,Jin Xiao,Marlise I. Klein,Megan L. Falsetta,Bingwen Lu,Claire M. Delahunty,John R. Yates,Arne Heydorn,Hyun Koo +8 more
TL;DR: The mechanisms through which the Streptococcus mutans-produced EPS-matrix modulates the three-dimensional architecture and the population shifts during morphogenesis of biofilms on a saliva-coated-apatitic surface are explored, illustrating the critical interaction between matrix architecture and pH heterogeneity in the 3D environment.
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