Higher copy numbers of the potato RB transgene correspond to enhanced transcript and late blight resistance levels.
James M. Bradeen,Massimo Iorizzo,Dimitre Mollov,John A. Raasch,Lara C Kramer,Benjamin P. Millett,Sandra Austin-Phillips,Jiming Jiang,Domenico Carputo +8 more
Reads0
Chats0
TLDR
The effects of the RB transgene on foliar late blight resistance in transgenic cultivated potato under field production conditions are examined and suggest resistance-gene transcript levels may have to surpass a threshold before triggering RNA silencing.Abstract:
Late blight of potato ranks among the costliest of crop diseases worldwide. Host resistance offers the best means for controlling late blight, but previously deployed single resistance genes have been short-lived in their effectiveness. The foliar blight resistance gene RB, previously cloned from the wild potato Solanum bulbocastanum, has proven effective in greenhouse tests of transgenic cultivated potato. In this study, we examined the effects of the RB transgene on foliar late blight resistance in transgenic cultivated potato under field production conditions. In a two-year replicated trial, the RB transgene, under the control of its endogenous promoter, provided effective disease resistance in various genetic backgrounds, including commercially prominent potato cultivars, without fungicides. RB copy numbers and transcript levels were estimated with transgene-specific assays. Disease resistance was enhanced as copy numbers and transcript levels increased. The RB gene, like many other disease resistance genes, is constitutively transcribed at low levels. Transgenic potato lines with an estimated 15 copies of the RB transgene maintain high RB transcript levels and were ranked among the most resistant of 57 lines tested. We conclude that even in these ultra-high copy number lines, innate RNA silencing mechanisms have not been fully activated. Our findings suggest resistance-gene transcript levels may have to surpass a threshold before triggering RNA silencing. Strategies for the deployment of RB are discussed in light of the current research.read more
Citations
More filters
Journal ArticleDOI
Pivoting the Plant Immune System from Dissection to Deployment
TL;DR: Research over the last 25 years has led to an increasingly clear conceptual understanding of the molecular components of the plant immune system, which means the means to begin development of durable (long-lasting) disease resistance beyond the limits imposed by conventional breeding and in a manner that will replace costly and unsustainable chemical controls.
Journal ArticleDOI
Understanding and exploiting late blight resistance in the age of effectors
Vivianne G. A. A. Vleeshouwers,Sylvain Raffaele,Jack H. Vossen,Nicolas Champouret,Ricardo Oliva,María Eugenia Segretin,Hendrik Rietman,Liliana M. Cano,Anoma Lokossou,G.J.T. Kessel,Mathieu A. Pel,Sophien Kamoun +11 more
TL;DR: Genome-wide catalogs of P. infestans effectors are available, enabling effectoromics approaches that accelerate R gene cloning and specificity profiling and monitoring effector allelic diversity in pathogen populations can assist in R gene deployment in agriculture.
Journal ArticleDOI
Potato and Tomato Late Blight Caused by Phytophthora infestans: An Overview of Pathology and Resistance Breeding
TL;DR: In this article, the authors summarize the recent achievements in better understanding of the P infestans pathogenesis, host-pathogen interactions, and the progress made in developing genetic resistance in potato and tomato.
Journal ArticleDOI
Engineering Pathogen Resistance in Crop Plants: Current Trends and Future Prospects
TL;DR: The socioeconomic and biological reasons for the paradox that although technically useful solutions now exist for providing transgenic disease resistance, very few new crops have been introduced to the global market are explored.
Journal ArticleDOI
Phytophthora infestans isolates lacking class I ipiO variants are virulent on Rpi-blb1 potato.
Nicolas Champouret,Klaas Bouwmeester,Hendrik Rietman,Theo van der Lee,Chris Maliepaard,Anika Heupink,Peter J. I. van de Vondervoort,Evert Jacobsen,Richard G. F. Visser,Edwin A. G. van der Vossen,Francine Govers,Vivianne G. A. A. Vleeshouwers +11 more
TL;DR: This study shows that profiling the variation of Avr-blb1 within a P. infestans population is instrumental for predicting the effectiveness of Rpi- Blb1-mediated resistance in potato.
References
More filters
Journal Article
Processing of gene expression data generated by quantitative real-time RT-PCR.
TL;DR: The Q-Gene software application is a tool to cope with complex quantitative real-time PCR experiments at a high-throughput scale and considerably expedites and rationalizes the experimental setup, data analysis, and data management while ensuring highest reproducibility.
Journal ArticleDOI
Housekeeping gene selection for real-time RT-PCR normalization in potato during biotic and abiotic stress
TL;DR: A study of the variability of expression of hsp20.2, shown to be implicated in late blight stress, was realized, highlighting the importance of the choice of internal controls in such experiments.
Journal ArticleDOI
Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight
Junqi Song,James M. Bradeen,S. Kristine Naess,John A. Raasch,Susan M. Wielgus,Geraldine T. Haberlach,Jia Liu,Hanhui Kuang,Sandra Austin-Phillips,C. Robin Buell,John P. Helgeson,Jiming Jiang +11 more
TL;DR: The cloning of the major resistance gene RB in S. bulbocastanum is reported by using a map-based approach in combination with a long-range (LR)-PCR strategy, demonstrating that LR-PCR is a valuable approach to isolate genes that cannot be maintained in the bacterial artificial chromosome system.
Journal ArticleDOI
Spontaneous variability of single isolates of Phytophthora infestans. I. Cultural variation
C. E. Caten,J. L. Jinks +1 more
TL;DR: Isolates established from single zoospores gave rise to as much variation in their asexual progenies as the original mass cultures, and subcultures established by single sporangia or single hyphal tips were much more uniform than zoospore cultures, although significant differences in growth rate could still be detected.
Journal ArticleDOI
R gene expression induced by a type-III effector triggers disease resistance in rice.
Keyu Gu,Bing Yang,Dongsheng Tian,Lifang Wu,Dong-Jiang Wang,Dong-Jiang Wang,C. Sreekala,Fan Yang,Fan Yang,Zhaoqing Chu,Zhaoqing Chu,Guo-Liang Wang,Guo-Liang Wang,Frank F. White,Zhongchao Yin +14 more
TL;DR: Xa 27 specificity towards incompatible pathogens involves the differential expression of the R gene in the presence of the AvrXa27 effector.
Related Papers (5)
Applied Biotechnology to Combat Late Blight in Potato Caused by Phytophthora Infestans
Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans.
Brian J. Haas,Sophien Kamoun,Sophien Kamoun,Michael C. Zody,Michael C. Zody,Rays H. Y. Jiang,Rays H. Y. Jiang,Robert E. Handsaker,Liliana M. Cano,Manfred Grabherr,Chinnappa D. Kodira,Chinnappa D. Kodira,Sylvain Raffaele,Trudy Torto-Alalibo,Trudy Torto-Alalibo,Tolga O. Bozkurt,Audrey M. V. Ah-Fong,Lucia Alvarado,Vicky L. Anderson,Miles R. Armstrong,Anna O. Avrova,Laura Baxter,Jim Beynon,Petra C. Boevink,Stephanie R. Bollmann,Jorunn I. B. Bos,Vincent Bulone,Guohong Cai,Cahid Cakir,James C. Carrington,Megan Chawner,Lucio Conti,Stefano Costanzo,Richard Ewan,Noah Fahlgren,Michael A. Fischbach,Johanna Fugelstad,Eleanor M. Gilroy,Sante Gnerre,Pamela J. Green,Laura J. Grenville-Briggs,John Griffith,Niklaus J. Grünwald,Karolyn Horn,Neil R. Horner,Chia-Hui Hu,Edgar Huitema,Dong-Hoon Jeong,Alexandra M. E. Jones,Jonathan D. G. Jones,Richard W. Jones,Elinor K. Karlsson,Sridhara G. Kunjeti,Kurt Lamour,Zhenyu Liu,Li-Jun Ma,Dan MacLean,Marcus C. Chibucos,Hayes McDonald,Jessica McWalters,Harold J. G. Meijer,William Morgan,Paul Morris,Carol A. Munro,Keith O'Neill,Keith O'Neill,Manuel D. Ospina-Giraldo,Andrés Pinzón,Leighton Pritchard,Bernard H Ramsahoye,Qinghu Ren,Silvia Restrepo,Sourav Roy,Ari Sadanandom,Alon Savidor,Sebastian Schornack,David C. Schwartz,Ulrike Schumann,Ben Schwessinger,Lauren Seyer,Ted Sharpe,Cristina Silvar,Jing Song,David J. Studholme,Sean M. Sykes,Marco Thines,Marco Thines,Peter J. I. van de Vondervoort,Vipaporn Phuntumart,Stephan Wawra,R. Weide,Joe Win,Carolyn A. Young,Shiguo Zhou,William E. Fry,Blake C. Meyers,Pieter van West,Jean B. Ristaino,Francine Govers,Paul R. J. Birch,Stephen C. Whisson,Howard S. Judelson,Chad Nusbaum +102 more