Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
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TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
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Journal ArticleDOI
Mammalian haploid stem cells: establishment, engineering and applications
TL;DR: Genetically edited AG-haESC lines may further serve as an ideal candidate for the establishment of a sperm bank, which is a highly cost-effective approach, and a wide range of engineered semi-cloned mice have been produced.
Journal ArticleDOI
A genome-wide CRISPR screen identifies regulation factors of the TLR3 signalling pathway
TL;DR: Depletion of AhR had a dual effect on the TLR3 response, abrogating IL-8 production and enhancing IP-10 release, and overall, these results reveal AhR plays a role in theTLR3 cellular innate immune response.
Journal ArticleDOI
Identification of Genes That Modulate Susceptibility to Formaldehyde and Imatinib by Functional Genomic Screening in Human Haploid KBM7 Cells
Hua Shen,Cliona M. McHale,Syed I. Haider,Cham Jung,Susie Zhang,Martyn T. Smith,Luoping Zhang +6 more
TL;DR: A semi-solid medium based screening approach that cultures individual mutant colonies from chemically resistant cells, faster (by 2-3 weeks) and with less labor than the original liquid medium-based approach is developed.
Book ChapterDOI
Using Functional Genetics in Haploid Cells for Drug Target Identification.
TL;DR: This chapter provides a step-by-step protocol for performing haploid gene trap screens, a powerful tool to identify targets for drug development as well as chemogenetic interactions.
Posted ContentDOI
Quantitative live-cell imaging yields novel insight into endogenous WNT/CTNNB1 signaling dynamics
TL;DR: This work combines CRISPR/Cas9-mediated genome editing and quantitative live-cell microscopy to measure diffusion characteristics of fluorescently tagged, endogenous CTNNB1 in human cells with high spatiotemporal resolution and reveals that WNT regulates the dynamic distribution of CTNNBs across different functional pools by modulating the destruction complex, nucleocytoplasmic shuttling and nuclear retention.
References
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Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Bypass of Senescence After Disruption of p21CIP1/WAF1 Gene in Normal Diploid Human Fibroblasts
TL;DR: At the checkpoint between the prereplicative phase of growth and the phase of chromosome replication, cells lacking p21 failed to arrest the cell cycle in response to DNA damage, but their apoptotic response and genomic stability were unaltered.
Journal Article
Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination
TL;DR: The c-myc null cell lines reported here are a new experimental system in which to investigate the importance of putative c-Myc target genes and to identify novel downstream genes involved in cell cycle progression and apoptosis.
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