Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
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TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
Citations
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Journal ArticleDOI
Genome‐wide screen for anticancer drug resistance in haploid human embryonic stem cells
Jonathan Nissenbaum,Mordecai Peretz,Tamar Golan-Lev,Rivki Cashman,Hagit Philip,Benjamin Reubinoff,Oded Kopper,Nissim Benvenisty +7 more
TL;DR: In this paper , the authors performed a genome-wide screen in haploid human embryonic stem cells (hESCs) to identify genes and pathways which can confer resistance to these drugs, demonstrating several common pathways.
Journal ArticleDOI
Revisited role of TRAF2 and TRAF2 C-terminal domain in endoplasmic reticulum stress-induced autophagy in HAP1 leukemia cells.
Camilla Palumbo,A. Mecchia,Alessio Bocedi,Katia Aquilano,Daniele Lettieri-Barbato,Marco Rosina,Almerinda Di Venere,Carlo Rodolfo,Anna Maria Caccuri +8 more
TL;DR: In this article , the authors investigated the involvement of TRAF2 in autophagy regulation in the human leukemic HAP1 cell line, under both basal and ER stress conditions.
Posted ContentDOI
Quantitative live-cell imaging and computational modelling yield novel insight into endogenous WNT/CTNNB1 signaling dynamics
TL;DR: In this paper, the authors combine CRISPR/Cas9-mediated genome editing and quantitative live-cell microscopy to measure diffusion characteristics of fluorescently tagged, endogenous CTNNB1 in human cells.
Journal ArticleDOI
The use of human induced pluripotent stem cells (hiPSC) for modeling blood disorders
Howard C.H. Chow,Alice M.S. Cheung,Ronald A. Li,Camie W.Y. Chan,Eric Chi Wai So,Anskar Y.H. Leung +5 more
TL;DR: This article will review issues with particular reference to the impact of human induced pluripotent cell (hiPSC) technology in the modeling of human blood disorders.
Book ChapterDOI
Genome Engineering Using Haploid Embryonic Stem Cells.
Takuro Horii,Izuho Hatada +1 more
TL;DR: Live mice can be successfully obtained by nuclear transplantation of haploid ES cells into oocytes, providing a novel approach for the generation of gene-modified animals.
References
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Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Bypass of Senescence After Disruption of p21CIP1/WAF1 Gene in Normal Diploid Human Fibroblasts
TL;DR: At the checkpoint between the prereplicative phase of growth and the phase of chromosome replication, cells lacking p21 failed to arrest the cell cycle in response to DNA damage, but their apoptotic response and genomic stability were unaltered.
Journal Article
Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination
TL;DR: The c-myc null cell lines reported here are a new experimental system in which to investigate the importance of putative c-Myc target genes and to identify novel downstream genes involved in cell cycle progression and apoptosis.
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