Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
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TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
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Journal ArticleDOI
Collection of homozygous mutant mouse embryonic stem cells arising from autodiploidization during haploid gene trap mutagenesis.
Ayako Yamanishi,Atsushi Matsuba,Ryohei Kondo,Rie Akamatsu,Sachiyo Tanaka,Masahiro Tokunaga,Kyoji Horie,Chikara Kokubu,Yasumasa Ishida,Junji Takeda +9 more
TL;DR: The results demonstrate the feasibility of the approach to provide an efficient platform for mutagenesis of ESCs and functional analysis of the mammalian genome.
Journal ArticleDOI
The repertoire of copy number alteration signatures in human cancer
Ziyu Tao,Shixiang Wang,Chenxu Wu,Tao Wu,Xiangyu Zhao,Wei Ning,Guangshuai Wang,Jinyu Wang,Jing Chen,Kaixuan Diao,Fuxiang Chen,Xue-Song Liu +11 more
TL;DR: Wang et al. as mentioned in this paper developed a mechanism-agnostic method to categorize CNA based on various fragment properties, which reflect the consequences of mutagenic processes and can be extracted from different types of data, including whole genome sequencing (WGS) and SNP array.
Journal ArticleDOI
Isobaric Tag‐Based Protein Profiling across Eight Human Cell Lines Using High‐Field Asymmetric Ion Mobility Spectrometry and Real‐Time Database Searching
TL;DR: The authors determine the relativeprotein abundance profiles of a panel of eight diverse, but commonly studied human cell lines and show that the differences in the relative protein abundance profiles reflect cell line diversity.
Patent
Pla2g16 as a target for antiviral compounds
TL;DR: In some aspects, the invention provides compositions and methods for inhibiting viral infection as mentioned in this paper. But this method is not suitable for identifying antiviral compounds, and it cannot be used to detect viruses.
Posted ContentDOI
Establishment of an eHAP1 Human Haploid Cell Line Hybrid Reference Genome Assembled from Short and Long Reads
TL;DR: The union of long and short reads demonstrates the utility in combining sequencing platforms to generate a high-quality reference genome de novo solely from low coverage data.
References
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Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Bypass of Senescence After Disruption of p21CIP1/WAF1 Gene in Normal Diploid Human Fibroblasts
TL;DR: At the checkpoint between the prereplicative phase of growth and the phase of chromosome replication, cells lacking p21 failed to arrest the cell cycle in response to DNA damage, but their apoptotic response and genomic stability were unaltered.
Journal Article
Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination
TL;DR: The c-myc null cell lines reported here are a new experimental system in which to investigate the importance of putative c-Myc target genes and to identify novel downstream genes involved in cell cycle progression and apoptosis.
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