Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
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TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
Citations
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Journal ArticleDOI
New tools for old drugs: Functional genetic screens to optimize current chemotherapy
TL;DR: This review focuses on novel advanced techniques to decipher the interplay between genetic alterations and drug response and expects these functional screens will enable the discovery of novel cancer-specific vulnerabilities, and through clinical validation, move the field of predictive biomarkers forward.
Book ChapterDOI
Functional Genomic Strategies for Elucidating Human–Virus Interactions: Will CRISPR Knockout RNAi and Haploid Cells?
TL;DR: Three screening approaches for elucidating host–virus interactions are compared, outline their key strengths and weaknesses including a comparison of an arrayed multiple orthologous RNAi reagent screen to a pooled CRISPR/Cas9 human rhinovirus 14–human cell interaction screen, and recount some notable insights made possible by each.
Journal ArticleDOI
Uncoordinated centrosome cycle underlies the instability of non-diploid somatic cells in mammals.
Kan Yaguchi,Takahiro Yamamoto,Ryo Matsui,Yuki Tsukada,Atsuko Shibanuma,Keiko Kamimura,Toshiaki Koda,Ryota Uehara +7 more
TL;DR: It is proposed that incompatibility between the centrosome duplication and DNA replication cycles arising from different scaling properties of these bioprocesses upon ploidy changes underlies the instability of non-diploid somatic cells in mammals.
Journal ArticleDOI
Single and Dual Vector Gene Therapy with AAV9-PHP.B Rescues Hearing in Tmc1 Mutant Mice.
Jason Wu,Paola Solanes,Carl Nist-Lund,Sofia Spataro,Olga Shubina-Oleinik,Irina Marcovich,Hannah Goldberg,Bernard L. Schneider,Jeffrey R. Holt +8 more
TL;DR: It is demonstrated that AAV9-PHP.B gene therapy can promote hair cell survival and successfully rescues hearing in three distinct mouse models of hearing loss.
Journal ArticleDOI
Haploid animal cells.
TL;DR: The potential use of haploid cells and puts haploid animal cells into a historical and biological context are examined and application of haploids in genetic screening holds promise for advancing the genetic exploration of mammalian genomes.
References
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Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Bypass of Senescence After Disruption of p21CIP1/WAF1 Gene in Normal Diploid Human Fibroblasts
TL;DR: At the checkpoint between the prereplicative phase of growth and the phase of chromosome replication, cells lacking p21 failed to arrest the cell cycle in response to DNA damage, but their apoptotic response and genomic stability were unaltered.
Journal Article
Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination
TL;DR: The c-myc null cell lines reported here are a new experimental system in which to investigate the importance of putative c-Myc target genes and to identify novel downstream genes involved in cell cycle progression and apoptosis.
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