Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
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TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
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Journal ArticleDOI
The genomic landscape of sensitivity to arsenic trioxide uncovered by genome-wide CRISPR-Cas9 screening
TL;DR: In this article , a genome-wide CRISPR-Cas9 knockdown screening system was constructed for ATO screening, and the results were subjected to pathway enrichment analysis using WebGestalt and KOBAS.
Pooled tagging and hydrophobic targeting of endogenous proteins for unbiased mapping of unfolded protein responses
TL;DR: In this paper , the authors used a hydrophobic HaloTag ligand to misfold tagged proteins, inducing spatially restricted proteotoxic stress that is read out by single cell RNA sequencing.
Book ChapterDOI
Higher-order Chromatin Organization in Diseases, from Chromosomal Position Effect to Phenotype Variegation
TL;DR: In this article , structural genomic variants disrupt genomic domains and how these disruptions result in diseases, and the authors discuss in detail the role of position effects on the chromatin architecture, gene regulation, and in turn, the fate of a cell.
Uncovering Bunyavirus-Host Interactions
TL;DR: In addition to discovering novel aspects of bunyavirus biology, both of these cellular pathways have FDA approved pharmacological inhibitors and future studies aim to determine whether these factors can serve as targets for antiviral intervention.
Reference EntryDOI
Haploid Embryonic Stem Cells
Ni Hong,Yunhan Hong +1 more
TL;DR: Haploidy in a vertebrate is able to support stable cell culture and pluripotency, with haploid ES cells elegantly combining haploidy and pluripsetency, and offering a unique yeast-like system for in vitro genetic analyses of molecular, cellular, and developmental events in various cell lineages.
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Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Bypass of Senescence After Disruption of p21CIP1/WAF1 Gene in Normal Diploid Human Fibroblasts
TL;DR: At the checkpoint between the prereplicative phase of growth and the phase of chromosome replication, cells lacking p21 failed to arrest the cell cycle in response to DNA damage, but their apoptotic response and genomic stability were unaltered.
Journal Article
Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination
TL;DR: The c-myc null cell lines reported here are a new experimental system in which to investigate the importance of putative c-Myc target genes and to identify novel downstream genes involved in cell cycle progression and apoptosis.
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