Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
Citations
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Journal ArticleDOI
High-throughput functional genomics using CRISPR–Cas9
TL;DR: A review of the latest applications of CRISPR-Cas9 in mammalian functional genomics screens is presented in this article, which covers related genome-scale applications of Cas9 for either gene knockout or transcriptional modulation.
Journal ArticleDOI
Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens
Jan E. Carette,Carla P. Guimaraes,Malini Varadarajan,Annie S. Park,Irene Wuethrich,Alzbeta Godarova,Maciej Kotecki,Brent H. Cochran,Eric Spooner,Hidde L. Ploegh,Thijn R. Brummelkamp +10 more
TL;DR: Using insertional mutagenesis to develop a screening method to generate null alleles in a human cell line haploid for all chromosomes except chromosome 8, host factors essential for infection with influenza and genes encoding important elements of the biosynthetic pathway of diphthamide, which are required for the cytotoxic effects ofdiphtheria toxin and exotoxin A are identified.
Journal ArticleDOI
Caspase-mediated cleavage of phospholipid flippase for apoptotic phosphatidylserine exposure
Katsumori Segawa,Sachiko Kurata,Yuichi Yanagihashi,Thijn R. Brummelkamp,Fumihiko Matsuda,Shigekazu Nagata +5 more
TL;DR: Using a haploid genetic screen in human cells, it is found that ATP11C and CDC50A are required for aminophospholipid translocation from the outer to the inner plasma membrane leaflet; that is, they display flippase activity.
Journal ArticleDOI
Genome-wide Maps of Nuclear Lamina Interactions in Single Human Cells
Jop Kind,Jop Kind,Ludo Pagie,Sandra S. de Vries,Leila Nahidiazar,Siddharth S. Dey,Magda Bienko,Ye Zhan,Bryan R. Lajoie,Carolyn A. de Graaf,Mario Amendola,Geoffrey Fudenberg,Maxim Imakaev,Leonid A. Mirny,Kees Jalink,Job Dekker,Alexander van Oudenaarden,Bas van Steensel +17 more
TL;DR: The consistency of NL contacts is inversely linked to gene activity in single cells and correlates positively with the heterochromatic histone modification H3K9me3, which highlights fundamental principles of single-cell chromatin organization.
Journal ArticleDOI
Obligate ligation-gated recombination (ObLiGaRe): custom-designed nuclease-mediated targeted integration through nonhomologous end joining.
TL;DR: ObLiGaRe is described, a new method for site-specific gene insertions that uses the efficient NHEJ pathway and acts independently of HR, and has enabled us to insert a 15-kb inducible gene expression cassette at a defined locus in human cell lines.
References
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Journal ArticleDOI
Stable Haploid Cultured Cell Lines from frog Embryos
TL;DR: These two haploid cell lines have been established from androgenetic embryos of the frog, Rana pipiens and are exceptional in their ability to compete successfully with the diploid variants which arise by endomitosis or cell fusion and which usually overgrow the haploid population.
Journal ArticleDOI
Near-haploid acute lymphoblastic leukemia: a unique subgroup with a poor prognosis?
TL;DR: Teenage girls with acute lymphoblastic leukemia whose leukemia cells were near-haploid may represent a unique subgroup of ALL with a poor prognosis, and cytogenetic analysis and flow cytometric analysis of DNA content are included in initial evaluation of patients with ALL.
Book
Molecular cell genetics
TL;DR: The Biology and Genetics of the Chinese Hamster Development of theChinese Hamster Ovary (CHO) Cell for Use in Somatic Cell Genetics and the Establishment of the V79 China Hamster Lung Cell Line are reviewed.
Journal ArticleDOI
Chromosome-wide event accompanies the expression of recessive mutations in tetraploid cells.
Lawrence A. Chasin,Gail Urlaub +1 more
TL;DR: Mutants resistant to 6-thioguanine were induced in pseudotetraploid hybrid Chinese hamster cells homozygous wild-type at the locus for hypoxanthine phophoribosyl transferase but heterozygous for the linked marker glucose-6-phosphate dehydrogenase.
Journal ArticleDOI
Ph-positive chronic myeloid leukemia with near-haploid conversion in vivo and establishment of a continuously growing cell line with similar cytogenetic pattern
Borje S. Andersson,Miloslav Beran,Sen Pathak,Angela Goodacre,Barthel Barlogie,Kenneth B. McCredie +5 more
TL;DR: Cytogenetic studies revealed the presence of two near-haploid cell populations with +8 and +8, +15, respectively, both of them containing the translocation t(9;22) in the original tumor cell sample.
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