Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
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TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
Citations
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Journal ArticleDOI
Fluorescence-based phenotypic selection allows forward genetic screens in haploid human cells.
Lidia M. Duncan,Richard T. Timms,Eszter Zavodszky,Florencia Cano,Gordon Dougan,Felix Randow,Paul J. Lehner +6 more
TL;DR: It is shown that selection for a cell surface phenotype via fluorescence-activated cell sorting can identify the key molecules in an intracellular pathway, in this case MHC class I antigen presentation.
Journal ArticleDOI
Disease-associated patterns of disomic chromosomes in hyperhaploid neoplasms.
TL;DR: The present findings indicate that retention of disomy for some chromosomes is pathogenetically important and that the chromosome(s) maintained in two copies is related to cell type or histological context.
Journal ArticleDOI
NUDT2 disruption elevates diadenosine tetraphosphate (Ap4A) and down-regulates immune response and cancer promotion genes
Andrew S. Marriott,Olga Vasieva,Yongxiang Fang,Nikki A. Copeland,Alexander G. McLennan,Nigel J. Jones +5 more
TL;DR: The results suggest that the NUDT2 protein could be a novel cancer chemotherapeutic target, with its inhibition potentially exerting strong anti-tumor effects via multiple pathways involving metastasis, invasion, immunosuppression and apoptosis.
Journal ArticleDOI
Functional genomic screening approaches in mechanistic toxicology and potential future applications of CRISPR-Cas9.
TL;DR: CRISPR-Cas9 is a powerful and flexible functional genomic screening approach that can be harnessed to provide unprecedented mechanistic insight in the field of modern toxicology.
Journal ArticleDOI
Medaka haploid embryonic stem cells are susceptible to Singapore grouper iridovirus as well as to other viruses of aquaculture fish species.
TL;DR: It is demonstrated that medaka haploid ES cells are susceptible to SGIV, as well as to SVCV and RGNNV, offering a unique opportunity for the identification of host factors by genetic screening.
References
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Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Bypass of Senescence After Disruption of p21CIP1/WAF1 Gene in Normal Diploid Human Fibroblasts
TL;DR: At the checkpoint between the prereplicative phase of growth and the phase of chromosome replication, cells lacking p21 failed to arrest the cell cycle in response to DNA damage, but their apoptotic response and genomic stability were unaltered.
Journal Article
Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination
TL;DR: The c-myc null cell lines reported here are a new experimental system in which to investigate the importance of putative c-Myc target genes and to identify novel downstream genes involved in cell cycle progression and apoptosis.
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