Journal ArticleDOI
Isolation and Characterization of a Near-Haploid Human Cell Line
TLDR
By serial subcloning, a near-haploid subclone (P1-55) is isolated from a heterogeneous human leukemia cell line, KBM-7, which has approximately half the human diploid DNA content and has a haploid karyotype except for a disomy of chromosome 8.About:
This article is published in Experimental Cell Research.The article was published on 1999-11-01. It has received 136 citations till now. The article focuses on the topics: Karyotype & Ploidy.read more
Citations
More filters
Journal ArticleDOI
Intracellular trafficking of retroviral genomes during the early phase of infection: viral exploitation of cellular pathways.
TL;DR: A deeper understanding of the pathways and components used by the different classes of retroviruses should enable the development of better and more efficient retroviral vectors for use in gene therapy protocols in vivo.
Journal ArticleDOI
A reversible gene trap collection empowers haploid genetics in human cells
Tilmann Bürckstümmer,Carina Banning,Philipp Hainzl,Richard Schobesberger,Claudia Kerzendorfer,Florian M. Pauler,Doris Chen,Nicole C.C. Them,Fiorella Schischlik,Manuele Rebsamen,Michal Smida,Ferran Fece de la Cruz,Ana Lapao,Melissa Liszt,Benjamin Eizinger,Philipp M. Guenzl,Vincent A. Blomen,Tomasz Konopka,Bianca V. Gapp,Katja Parapatics,Barbara B. Maier,Barbara B. Maier,Johannes Stöckl,Wolfgang Fischl,Sejla Salic,M Rita Taba Casari,Sylvia Knapp,Sylvia Knapp,Keiryn L. Bennett,Christoph Bock,Jacques Colinge,Robert Kralovics,Gustav Ammerer,Georg Casari,Thijn R. Brummelkamp,Thijn R. Brummelkamp,Giulio Superti-Furga,Sebastian M.B. Nijman +37 more
TL;DR: This work established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions, covering 3,396 genes, one-third of the expressed genome, is DNA-barcoded.
Journal ArticleDOI
Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes
Hui Yang,Zhen Liu,Yu Ma,Cuiqing Zhong,Qi Yin,Chikai Zhou,Chikai Zhou,Linyu Shi,Yijun Cai,Hanzhi Zhao,Hui Wang,Fan Tang,Yan Wang,Chen-Chen Zhang,Xinyuan Liu,Dongmei Lai,Ying Jin,Qiang Sun,Jinsong Li +18 more
TL;DR: The derivation of haESCs from parthenogenetic blastocysts of Macaca fascicularis monkeys is reported, demonstrating that PG-haESCs can be generated from monkeys, providing an ideal tool for genetic analyses in primates.
Journal ArticleDOI
Genetic modification and screening in rat using haploid embryonic stem cells.
Wei Li,Xin Li,Xin Li,Tianda Li,Minggui Jiang,Minggui Jiang,Haifeng Wan,Guan-Zheng Luo,Chunjing Feng,Xiaolong Cui,Fei Teng,Yan Yuan,Yan Yuan,Quan Zhou,Quan Zhou,Qi Gu,Qi Gu,Ling Shuai,Ling Shuai,Jiahao Sha,Yamei Xiao,Liu Wang,Zhonghua Liu,Xiu-Jie Wang,Xiaoyang Zhao,Qi Zhou +25 more
TL;DR: The derivation of rat androgenetic haploid embryonic stem cells (RahESCs) are reported, which have 21 chromosomes, express pluripotency markers, differentiate into three germ layer cells, and contribute to the germline.
Journal ArticleDOI
Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2.
Iva A. Tchasovnikarova,Richard T. Timms,Christopher H. Douse,Rhys C. Roberts,Gordon Dougan,Robert E. Kingston,Yorgo Modis,Paul J. Lehner +7 more
TL;DR: A novel method is exploited, differential viral accessibility (DIVA), to show that loss of MORC2 results in chromatin decompaction at these target loci, which is concomitant with a loss of H3K9me3 deposition and transcriptional derepression, and define a critical role for MORC 2 in epigenetic silencing by the HUSH complex.
References
More filters
Journal ArticleDOI
Altering the genome by homologous recombination.
TL;DR: The current status of gene targeting with particular emphasis on germ line modification of the mouse genome is discussed, and the different methods so far employed to identify those rare embryonic stem cells in which the desired targeting event has occurred are described.
Journal ArticleDOI
Production of clones of homozygous diploid zebra fish ( Brachydanio rerio )
TL;DR: Clones of homozygous fish have been produced from individual homozygotes and associated genetic methods facilitate genetic analyses of this vertebrate.
Journal ArticleDOI
Bypass of Senescence After Disruption of p21CIP1/WAF1 Gene in Normal Diploid Human Fibroblasts
TL;DR: At the checkpoint between the prereplicative phase of growth and the phase of chromosome replication, cells lacking p21 failed to arrest the cell cycle in response to DNA damage, but their apoptotic response and genomic stability were unaltered.
Journal Article
Phenotypes of c-Myc-deficient rat fibroblasts isolated by targeted homologous recombination
TL;DR: The c-myc null cell lines reported here are a new experimental system in which to investigate the importance of putative c-Myc target genes and to identify novel downstream genes involved in cell cycle progression and apoptosis.
Related Papers (5)
Ebola virus entry requires the cholesterol transporter Niemann–Pick C1
Jan E. Carette,Jan E. Carette,Matthijs Raaben,Anthony C. Wong,Andrew S. Herbert,Gregor Obernosterer,Gregor Obernosterer,Nirupama Mulherkar,Ana I. Kuehne,Philip J. Kranzusch,April M. Griffin,Gordon Ruthel,Paola Dal Cin,John M. Dye,Sean P. J. Whelan,Kartik Chandran,Thijn R. Brummelkamp,Thijn R. Brummelkamp +17 more
Forward and Reverse Genetics through Derivation of Haploid Mouse Embryonic Stem Cells
Ulrich Elling,Jasmin Taubenschmid,Gerald Wirnsberger,Ronan C. O'Malley,Simon-Pierre Demers,Quentin Vanhaelen,Andrey I. Shukalyuk,Gerald Schmauss,Daniel Schramek,Frank Schnuetgen,Harald von Melchner,Joseph R. Ecker,Joseph R. Ecker,William L. Stanford,William L. Stanford,William L. Stanford,Johannes Zuber,Alexander Stark,Josef M. Penninger +18 more