miRNAs in lung cancer - Studying complex fingerprints in patient's blood cells by microarray experiments
Andreas Keller,Petra Leidinger,Anne Borries,Anke Wendschlag,Frank Wucherpfennig,Matthias Scheffler,Hanno Huwer,Hans-Peter Lenhof,Eckart Meese +8 more
TLDR
The findings support the idea that neoplasia may lead to a deregulation of miRNA expression in blood cells of cancer patients compared toBlood cells of healthy individuals and provide evidence that miRNA patterns can be used to detect human cancers from blood cells.Abstract:
Deregulated miRNAs are found in cancer cells and recently in blood cells of cancer patients. Due to their inherent stability miRNAs may offer themselves for blood based tumor diagnosis. Here we addressed the question whether there is a sufficient number of miRNAs deregulated in blood cells of cancer patients to be able to distinguish between cancer patients and controls. We synthesized 866 human miRNAs and miRNA star sequences as annotated in the Sanger miRBase onto a microarray designed by febit biomed gmbh. Using the fully automated Geniom Real Time Analyzer platform, we analyzed the miRNA expression in 17 blood cell samples of patients with non-small cell lung carcinomas (NSCLC) and in 19 blood samples of healthy controls. Using t-test, we detected 27 miRNAs significantly deregulated in blood cells of lung cancer patients as compared to the controls. Some of these miRNAs were validated using qRT-PCR. To estimate the value of each deregulated miRNA, we grouped all miRNAs according to their diagnostic information that was measured by Mutual Information. Using a subset of 24 miRNAs, a radial basis function Support Vector Machine allowed for discriminating between blood cellsamples of tumor patients and controls with an accuracy of 95.4% [94.9%-95.9%], a specificity of 98.1% [97.3%-98.8%], and a sensitivity of 92.5% [91.8%-92.5%]. Our findings support the idea that neoplasia may lead to a deregulation of miRNA expression in blood cells of cancer patients compared to blood cells of healthy individuals. Furthermore, we provide evidence that miRNA patterns can be used to detect human cancers from blood cells.read more
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Journal ArticleDOI
MicroRNA: Biogenesis, Function and Role in Cancer.
TL;DR: The P-body model outlines microRNA sorting and shuttling between specialized P- body compartments that house enzymes required for slicer –dependent and –independent silencing, addressing the reversibility of these silencing mechanisms.
Journal ArticleDOI
Circulating microRNAs: Association with disease and potential use as biomarkers
TL;DR: The disease-specific profiles of circulating microRNAs, and the methodologies used for their detection and quantification are reviewed, and possible functions of circulatingmicroRNAs and their potential as non-invasive biomarkers are discussed.
Journal ArticleDOI
Toward the blood-borne miRNome of human diseases
Andreas Keller,Petra Leidinger,Andrea S. Bauer,Abdou ElSharawy,Jan Haas,Christina Backes,Anke Wendschlag,Nathalia Giese,Christine Tjaden,Katja Ott,Jens Werner,Thilo Hackert,Klemens Ruprecht,Hanno Huwer,Junko Huebers,Gunnar Jacobs,Philip Rosenstiel,Henrik Dommisch,Arne S. Schaefer,Joachim Müller-Quernheim,Bernd Wullich,Bastian Keck,Norbert Graf,Joerg Reichrath,Britta Vogel,Almut Nebel,Sven Uwe Jager,Peer F Staehler,Ioannis Amarantos,Valesca Boisguerin,Cord F Staehler,Markus Beier,Matthias Scheffler,Markus W. Büchler,J Wischhusen,Sebastian F M Haeusler,Johannes Dietl,Sylvia Hofmann,Hans-Peter Lenhof,Stefan Schreiber,Hugo A. Katus,Wolfgang Rottbauer,Benjamin Meder,Joerg Hoheisel,Andre Franke,Eckart Meese +45 more
TL;DR: In a multicenter study, the expression profiles of 863 microRNAs were determined by array analysis of 454 blood samples from human individuals with different cancers or noncancer diseases, and this 'miRNome' was validated by quantitative real-time PCR.
Journal ArticleDOI
miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer
Kristen M. Foss,Chao Sima,Donatella Ugolini,Donatella Ugolini,Monica Neri,Kristi Allen,Glen J. Weiss +6 more
TL;DR: Serum miRNAs are differentially expressed between patients with early-stage NSCLC and controls, and the utility of miR-1254 andmiR-574-5p serum-based biomarkers as minimally invasive screening and triage tools for subsequent diagnostic evaluation warrants additional validation.
Journal ArticleDOI
MicroRNAs in cerebrospinal fluid identify glioblastoma and metastatic brain cancers and reflect disease activity.
Nadiya M. Teplyuk,Brit Mollenhauer,Galina Gabriely,Alf Giese,Ella Kim,Michael Smolsky,Ryan Y. Kim,Marlon Garzo Saria,Sandra Pastorino,Santosh Kesari,Anna M. Krichevsky +10 more
TL;DR: This study demonstrates that microRNA-based detection of brain malignancies can be reliably performed and that microRNAs in CSF can serve as biomarkers of treatment response in brain cancers.
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