NEAT1 Long Noncoding RNA and Paraspeckle Bodies Modulate HIV-1 Posttranscriptional Expression
TLDR
The knockdown of NEAT1 enhances virus production through increased nucleus-to-cytoplasm export of Rev-dependent instability element (INS)-containing HIV-1 mRNAs as well as identifying the nuclear paraspeckle body as another important subcellular organelle for HIV- 1 replication.Abstract:
Most of the human genome is transcribed into protein-noncoding RNAs (ncRNAs), including small ncRNAs and long ncRNAs (lncRNAs). Over the past decade, rapidly emerging evidence has increasingly supported the view that lncRNAs serve key regulatory and functional roles in mammal cells. HIV-1 replication relies on various cell functions. To date, while the involvement of host protein factors and microRNAs (miRNAs) in the HIV-1 life cycle has been extensively studied, the relationship between lncRNAs and HIV-1 remains uncharacterized. Here, we have profiled 83 disease-related lncRNAs in HIV-1-infected T cells. We found NEAT1 to be one of several lncRNAs whose expression is changed by HIV-1 infection, and we have characterized its role in HIV-1 replication. We In the abstract, added definition of INS OK, or should "cis-acting" be added?report here that the knockdown of NEAT1 enhances virus production through increased nucleus-to-cytoplasm export of Rev-dependent instability element (INS)-containing HIV-1 mRNAs. IMPORTANCE Long protein-noncoding RNAs (lncRNAs) play roles in regulating gene expression and modulating protein activities. There is emerging evidence that lncRNAs are involved in the replication of viruses. To our knowledge, this report is the first to characterize a role contributed by an lncRNA, NEAT1, to HIV-1 replication. NEAT1 is essential for the integrity of the nuclear paraspeckle substructure. Based on our findings from NEAT1 knockdown, we have identified the nuclear paraspeckle body as another important subcellular organelle for HIV-1 replication.read more
Citations
More filters
Journal ArticleDOI
Long Noncoding RNA NEAT1-Dependent SFPQ Relocation from Promoter Region to Paraspeckle Mediates IL8 Expression upon Immune Stimuli
Katsutoshi Imamura,Naoto Imamachi,Gen Akizuki,Michiko Kumakura,Atsushi Kawaguchi,Kyosuke Nagata,Akihisa Kato,Yasushi Kawaguchi,Hiroki Sato,Misako Yoneda,Chieko Kai,Tetsushi Yada,Yutaka Suzuki,Toshimichi Yamada,Takeaki Ozawa,Kiyomi Kaneki,Tsuyoshi Inoue,Mika Kobayashi,Tatsuhiko Kodama,Youichiro Wada,Kazuhisa Sekimizu,Nobuyoshi Akimitsu +21 more
TL;DR: Nuclear enriched abundant transcript 1 (NEAT1), an essential lncRNA for the formation of nuclear body paraspeckles, is induced by influenza virus and herpes simplex virus infection as well as by Toll-like receptor3-p38 pathway-triggered poly I:C stimulation, resulting in excess formation of paraspeakles.
Journal ArticleDOI
Long Non-Coding RNA in the Pathogenesis of Cancers.
TL;DR: The latest findings regarding the roles and mechanisms of some important lncRNAs in the pathogenesis of certain malignant cancers, including lung, breast, liver, and colorectal cancers, as well as hematological malignancies and neuroblastoma are discussed.
Journal ArticleDOI
Long non-coding RNAs and complex diseases: from experimental results to computational models
TL;DR: Some state-of-the-art computational models are introduced, which could be effectively used to identify disease-related lncRNAs on a large scale and select the most promising disease- related lnc RNAs for experimental validation and discussed the future directions of developing computational models for lncRNA research.
Journal ArticleDOI
Identification of regulatory networks in HSCs and their immediate progeny via integrated proteome, transcriptome, and DNA methylome analysis
Nina Cabezas-Wallscheid,Daniel Klimmeck,Daniel Klimmeck,Jenny Hansson,Daniel B. Lipka,Alejandro Reyes,Qi Wang,Dieter Weichenhan,Amelie Lier,Lisa von Paleske,Simon Renders,Peer Wünsche,Petra Zeisberger,David Brocks,Lei Gu,Lei Gu,Carl Herrmann,Simon Haas,Marieke A.G. Essers,Benedikt Brors,Roland Eils,Wolfgang Huber,Michael D. Milsom,Christoph Plass,Jeroen Krijgsveld,Andreas Trumpp +25 more
TL;DR: An unexpectedly dynamic pattern of transcript isoform regulation is found, suggesting a critical regulatory role during HSC differentiation, and a cell cycle/DNA repair signature associated with multipotency in MPP2 cells is found.
Journal ArticleDOI
Long Non-Coding RNAs in the Regulation of Gene Expression: Physiology and Disease
Juliane Cristina Ribeiro Fernandes,Stephanie Maia Acuña,Juliana Ide Aoki,Lucile Maria Floeter-Winter,Sandra Marcia Muxel +4 more
TL;DR: Key aspects of lncRNA biology are reviewed, focusing on their role as regulatory elements in gene expression modulation during physiological and disease processes, with implications in host and pathogens physiology, and their role in immune response modulation.
References
More filters
Journal ArticleDOI
Modulation of HIV-1-host interaction: role of the Vpu accessory protein
TL;DR: This review will mostly focus on recent advances on the role of Vpu in CD4 downregulation and Tetherin antagonism and will discuss how these two functions may have impacted primate immunodeficiency virus cross-species transmission and the emergence of pandemic strain of HIV-1.
Journal ArticleDOI
PSF acts through the human immunodeficiency virus type 1 mRNA instability elements to regulate virus expression.
Andrei S. Zolotukhin,Daniel Michalowski,Jenifer Bear,Sergey Smulevitch,Abdulmaged M. Traish,Rui Peng,James G. Patton,Ivan N. Shatsky,Barbara K. Felber +8 more
TL;DR: It is shown that the INS region in HIV-1 gag mRNA is a high-affinity ligand of p54nrb/PSF, a heterodimeric transcription/splicing factor, and likely define a novel mRNA regulatory pathway that is hijacked byAIDS.
Journal ArticleDOI
Suppression of HIV-1 replication by microRNA effectors.
Christine Chable-Bessia,Oussama Meziane,Daniel Latreille,Robinson Triboulet,Alessia Zamborlini,Alexandre Wagschal,Jean-Marc Jacquet,Jacques Reynes,Yves Levy,Ali Saïb,Yamina Bennasser,Monsef Benkirane +11 more
TL;DR: It is shown that HIV-1 mRNAs associate and co-localize with components of the RNA Induced Silencing Complex (RISC), and some of the proteins required for miRNA-mediated silencing (miRNA effectors) are characterized.
Journal ArticleDOI
Identification and characterization of a virus-inducible non-coding RNA in mouse brain
TL;DR: Northern blotting of nuclear and cytoplasmic RNAs revealed that VINC is localized primarily in the nucleus of RAG cells and is thus a novel member of the nuclear ncRNA family.
Journal ArticleDOI
Nuclear RNA Export and Packaging Functions of HIV-1 Rev Revisited
TL;DR: It is shown that interference with the formation of an inhibitory ribonucleoprotein complex in the nucleus could lead to enhanced accessibility of the cytoplasmic HIV-1 RNA for translation and encapsidation, which might explain why Rev and tethered TAP exert the same pattern of pleiotropic effects.
Related Papers (5)
Chromatin signature reveals over a thousand highly conserved large non-coding RNAs in mammals
Mitchell Guttman,Ido Amit,Manuel Garber,Courtney French,Michael F. Lin,David M. Feldser,Maite Huarte,Maite Huarte,Or Zuk,Bryce W. Carey,John P. Cassady,Moran N. Cabili,Rudolf Jaenisch,Tarjei S. Mikkelsen,Tyler Jacks,Nir Hacohen,Bradley E. Bernstein,Bradley E. Bernstein,Manolis Kellis,Manolis Kellis,Aviv Regev,John L. Rinn,John L. Rinn,John L. Rinn,Eric S. Lander +24 more
The GENCODE v7 catalog of human long noncoding RNAs: analysis of their gene structure, evolution, and expression.
Thomas Derrien,Rory Johnson,Giovanni Bussotti,Andrea Tanzer,Sarah Djebali,Hagen Tilgner,Gregory Guernec,David C. Martin,Angelika Merkel,David G. Knowles,Julien Lagarde,Lavanya Veeravalli,Xiaoan Ruan,Yijun Ruan,Timo Lassmann,Piero Carninci,James B. Brown,Leonard Lipovich,José M. González,Mark G. Thomas,Carrie A. Davis,Ramin Shiekhattar,Thomas R. Gingeras,Tim Hubbard,Cedric Notredame,Jennifer Harrow,Roderic Guigó +26 more