NEAT1 Long Noncoding RNA and Paraspeckle Bodies Modulate HIV-1 Posttranscriptional Expression
TLDR
The knockdown of NEAT1 enhances virus production through increased nucleus-to-cytoplasm export of Rev-dependent instability element (INS)-containing HIV-1 mRNAs as well as identifying the nuclear paraspeckle body as another important subcellular organelle for HIV- 1 replication.Abstract:
Most of the human genome is transcribed into protein-noncoding RNAs (ncRNAs), including small ncRNAs and long ncRNAs (lncRNAs). Over the past decade, rapidly emerging evidence has increasingly supported the view that lncRNAs serve key regulatory and functional roles in mammal cells. HIV-1 replication relies on various cell functions. To date, while the involvement of host protein factors and microRNAs (miRNAs) in the HIV-1 life cycle has been extensively studied, the relationship between lncRNAs and HIV-1 remains uncharacterized. Here, we have profiled 83 disease-related lncRNAs in HIV-1-infected T cells. We found NEAT1 to be one of several lncRNAs whose expression is changed by HIV-1 infection, and we have characterized its role in HIV-1 replication. We In the abstract, added definition of INS OK, or should "cis-acting" be added?report here that the knockdown of NEAT1 enhances virus production through increased nucleus-to-cytoplasm export of Rev-dependent instability element (INS)-containing HIV-1 mRNAs. IMPORTANCE Long protein-noncoding RNAs (lncRNAs) play roles in regulating gene expression and modulating protein activities. There is emerging evidence that lncRNAs are involved in the replication of viruses. To our knowledge, this report is the first to characterize a role contributed by an lncRNA, NEAT1, to HIV-1 replication. NEAT1 is essential for the integrity of the nuclear paraspeckle substructure. Based on our findings from NEAT1 knockdown, we have identified the nuclear paraspeckle body as another important subcellular organelle for HIV-1 replication.read more
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The c-Myc-regulated lncRNA NEAT1 and paraspeckles modulate imatinib-induced apoptosis in CML cells.
Chengwu Zeng,Sichu Liu,Shuai Lu,Xibao Yu,Jing Lai,Yifan Wu,Shaohua Chen,Liang Wang,Zhi Yu,Gengxin Luo,Yangqiu Li +10 more
TL;DR: This study found that nuclear-enriched abundant transcript 1 (NEAT1), a lncRNA essential for the formation of nuclear body paraspeckles, is significantly repressed in primary CML cells and demonstrated that NEAT1 is regulated by c-Myc, and revealed that SFPQ regulates cell growth and death pathway-related genes, confirming its function in IM-induced apoptosis.
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Long noncoding RNA EGOT negatively affects the antiviral response and favors HCV replication.
Elena Carnero,Marina Barriocanal,Celia Prior,Juan Pablo Unfried,Victor Segura,Elizabeth Guruceaga,Mónica Enguita,Cristian Smerdou,Pablo Gastaminza,Puri Fortes +9 more
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Long Non-Coding RNA BST2/BISPR is Induced by IFN and Regulates the Expression of the Antiviral Factor Tetherin
TL;DR: It is proposed that several lncRNAs could be activated by IFN to control the potency of the antiviral IFN response, and lncBST2 has been renamed BISPR, from BST2 IFN-stimulated positive regulator.
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FMLNCSIM: fuzzy measure-based lncRNA functional similarity calculation model
TL;DR: A Fuzzy Measure-based LNCRNA functional SIMilarity calculation model (FMLNCSIM), based on the assumption that functionally similar lncRNAs tend to be associated with similar diseases, is developed and combined with the previously proposed model of Laplacian Regularized Least Squares for lncRNA-Disease Association (LRLSLDA).
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TL;DR: Research into lncRNAs in infected cells has already helped in the identification of novel cellular pathways and may help in the development of therapeutic tools for the treatment of viral infections, autoimmune diseases, neurological disorders and cancer.
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TL;DR: The results of an international collaboration to produce and make freely available a draft sequence of the human genome are reported and an initial analysis is presented, describing some of the insights that can be gleaned from the sequence.
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TL;DR: Three potentially functional classes of RNAs have been identified, two of which are syntenically conserved and correlate with the expression state of protein-coding genes and support a highly interleaved organization of the human transcriptome.
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