NEAT1 Long Noncoding RNA and Paraspeckle Bodies Modulate HIV-1 Posttranscriptional Expression
TLDR
The knockdown of NEAT1 enhances virus production through increased nucleus-to-cytoplasm export of Rev-dependent instability element (INS)-containing HIV-1 mRNAs as well as identifying the nuclear paraspeckle body as another important subcellular organelle for HIV- 1 replication.Abstract:
Most of the human genome is transcribed into protein-noncoding RNAs (ncRNAs), including small ncRNAs and long ncRNAs (lncRNAs). Over the past decade, rapidly emerging evidence has increasingly supported the view that lncRNAs serve key regulatory and functional roles in mammal cells. HIV-1 replication relies on various cell functions. To date, while the involvement of host protein factors and microRNAs (miRNAs) in the HIV-1 life cycle has been extensively studied, the relationship between lncRNAs and HIV-1 remains uncharacterized. Here, we have profiled 83 disease-related lncRNAs in HIV-1-infected T cells. We found NEAT1 to be one of several lncRNAs whose expression is changed by HIV-1 infection, and we have characterized its role in HIV-1 replication. We In the abstract, added definition of INS OK, or should "cis-acting" be added?report here that the knockdown of NEAT1 enhances virus production through increased nucleus-to-cytoplasm export of Rev-dependent instability element (INS)-containing HIV-1 mRNAs. IMPORTANCE Long protein-noncoding RNAs (lncRNAs) play roles in regulating gene expression and modulating protein activities. There is emerging evidence that lncRNAs are involved in the replication of viruses. To our knowledge, this report is the first to characterize a role contributed by an lncRNA, NEAT1, to HIV-1 replication. NEAT1 is essential for the integrity of the nuclear paraspeckle substructure. Based on our findings from NEAT1 knockdown, we have identified the nuclear paraspeckle body as another important subcellular organelle for HIV-1 replication.read more
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Downregulation of lncRNA NEAT1 Ameliorates LPS-Induced Inflammatory Responses by Promoting Macrophage M2 Polarization via miR-125a-5p/TRAF6/TAK1 Axis
Wei Wang,Wei Wang,Zhen-Hui Guo +2 more
TL;DR: In this paper, the role of NEAT1 in regulating macrophage polarization and its potential for alleviating inflammatory responses during sepsis pathogenesis was investigated in mouse RAW264.7 cells.
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The Opening of Pandora's Box: An Emerging Role of Long Noncoding RNA in Viral Infections.
TL;DR: Research into lncRNA will help identify novel viral infection-related regulators and design preventative and therapeutic strategies against virus-related diseases and immune disorders.
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Deep Sequencing of HIV-Infected Cells: Insights into Nascent Transcription and Host-Directed Therapy
Xinxia Peng,Pavel Sova,Richard Green,Matthew J. Thomas,Marcus J. Korth,Sean Proll,Jiabao Xu,Yanbing Cheng,Kang Yi,Li Chen,Zhiyu Peng,Jun Wang,Robert E. Palermo,Michael G. Katze +13 more
TL;DR: Mass sequencing is used to identify genes differentially expressed in CD4+ T cells during HIV-1 infection and shows that it is possible to identify candidate drugs for host-directed therapy against HIV/AIDS using the genomics-based approach.
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BPLLDA: Predicting lncRNA-Disease Associations Based on Simple Paths With Limited Lengths in a Heterogeneous Network.
Xiaofang Xiao,Wen Zhu,Bo Liao,Bo Liao,Junlin Xu,Changlong Gu,Binbin Ji,Yuhua Yao,Lihong Peng,Jialiang Yang,Jialiang Yang +10 more
TL;DR: Compared to existing methods, BPLLDA has a few advantages, including not demanding negative samples and the ability to predict associations related to novel lncRNAs or novel diseases, and may contribute to the understanding of lncRNA-associated diseases like certain cancers.
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The Long Noncoding RNA HEAL Regulates HIV-1 Replication through Epigenetic Regulation of the HIV-1 Promoter
Ti-Chun Chao,Qiong Zhang,Zhonghan Li,Shashi Kant Tiwari,Yue Qin,Edwin Yau,Ana B. Sanchez,Gatikrushna Singh,Kung-Yen Chang,Marcus Kaul,Marcus Kaul,Maile Y. Karris,Tariq M. Rana +12 more
TL;DR: The results suggest that silencing of HEAL or perturbation of the HEAL-FUS ribonucleoprotein complex could provide a new epigenetic silencing strategy to eradicate viral reservoirs and effect a cure for HIV-1/AIDS.
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TL;DR: The results of an international collaboration to produce and make freely available a draft sequence of the human genome are reported and an initial analysis is presented, describing some of the insights that can be gleaned from the sequence.
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TL;DR: Three potentially functional classes of RNAs have been identified, two of which are syntenically conserved and correlate with the expression state of protein-coding genes and support a highly interleaved organization of the human transcriptome.
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