Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
Understanding the Effect of tert-Butanol on Candida antarctica Lipase B Using Molecular Dynamics Simulations.
TL;DR: The effects of tert-butanol on the structure of CalB were investigated by MD simulations and the overall flexibility was increased and the distance between the catalytic residues ofCalB in tert- butanol indicated a higher likelihood of forming hydrogen bonds.
Journal ArticleDOI
2D regional correlation analysis of single-molecule time trajectories.
Xuefei Wang,H. Peter Lu +1 more
TL;DR: An application of this approach to analyze single-molecule fluorescence resonance energy transfer (FRET) fluctuation dynamics where the fluctuations are often complex is discussed, although this approach can be useful for analyzing other types of fluctuation Dynamics of various physical variables as well.
Journal ArticleDOI
On the relationships between kinetic schemes and two-state single molecule trajectories.
Ophir Flomenbom,Joseph Klafter +1 more
TL;DR: The binned successive waiting times PDFs are shown to be not only more robust than other functions when analyzing finite trajectories, but contain, in most cases, more information about the underlying kinetic scheme thanother functions in the limit of infinitely long trajectories.
Journal ArticleDOI
Low-temperature single-molecule spectroscopy on photosynthetic pigment–protein complexes from purple bacteria
Silke Oellerich,Jürgen Köhler +1 more
TL;DR: This review provides an overview of how the character of the electronically excited states of these pigment–protein complexes are determined by quantum mechanics and how the respective spectral signatures can be observed by single-molecule spectroscopy.
Journal ArticleDOI
Adaptation of Extremophilic Proteins with Temperature and Pressure: Evidence from Initiation Factor 6.
Paolo Calligari,Vania Calandrini,Jacques Ollivier,Jean-Baptiste Artero,Michael Härtlein,Mark A. Johnson,Gerald R. Kneller +6 more
TL;DR: It is shown for the first time, to the authors' knowledge, that the very common pattern of corresponding states for thermophilic protein adaptation can be extended to thermo-barophilic proteins.
References
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TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
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Rudolph A. Marcus,Norman Sutin +1 more
TL;DR: In this paper, the electron transfer reactions between ions and molecules in solution have been the subject of considerable experimental study during the past three decades, including charge transfer, photoelectric emission spectra, chemiluminescent electron transfer, and electron transfer through frozen media.
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Formation of glasses from liquids and biopolymers.
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The energy landscapes and motions of proteins.
TL;DR: The concepts that emerge from studies of the conformational substates and the motions between them permit a quantitative discussion of one simple reaction, the binding of small ligands such as carbon monoxide to myoglobin.
Journal ArticleDOI
Fluorescence spectroscopy of single biomolecules.
TL;DR: The progress in applying single-molecule detection and single-Molecule spectroscopy at room temperature by laser-induced fluorescence with the use of fluorophores that are site-specifically attached to macromolecules is reviewed.