Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Dissertation
From Brownian motion to supercooled water in confinements - a molecular dynamics simulation study
TL;DR: In this paper, the authors used power-law waiting time distributions to describe motion in a harmonic potential of water in confinements and found that the surface strongly affects the structure of water.
Journal ArticleDOI
Tracking structural transitions of bovine serum albumin in surfactant solutions by fluorescence correlation spectroscopy and fluorescence lifetime analysis
Xuzhu Zhang,Andrzej Poniewierski,Sen Hou,Krzysztof Sozanski,Agnieszka Wisniewska,Stefan A. Wieczorek,Tomasz Kalwarczyk,Lili Sun,Robert Hołyst +8 more
TL;DR: Fluorescence correlation spectroscopy is employed to track the structural transition of bovine serum albumin in low concentrated cationic, anionic, and nonionic surfactant solutions and identifies whether the structural change of protein results from its self-aggregation or unfolding.
Journal ArticleDOI
Anomalous lineshapes and aging effects in two-dimensional correlation spectroscopy
František Šanda,Shaul Mukamel +1 more
TL;DR: The signatures of anomalous relaxation in two-dimensional four wave mixing signals are analyzed using the continuous time random walk model for spectral diffusion to analyze their optical response to sequences of ultrashort optical pulse.
Journal ArticleDOI
Stretching of single poly-ubiquitin molecules revisited: dynamic disorder in the non-exponential unfolding kinetics.
TL;DR: This work suggests a sound and plausible alternative interpretation for the non-exponential kinetics in the stretching of poly-ubiquitin molecules, associated with dynamic disorder.
Journal ArticleDOI
Picosecond fluorescence dynamics of tryptophan and 5-fluorotryptophan in monellin: slow water-protein relaxation unmasked.
TL;DR: In this article, 5-fluorotryptophan (5Ftrp) was used to suppress pseudo-TDFSS in monellin and 5-trp-monellin.
References
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TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
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Rudolph A. Marcus,Norman Sutin +1 more
TL;DR: In this paper, the electron transfer reactions between ions and molecules in solution have been the subject of considerable experimental study during the past three decades, including charge transfer, photoelectric emission spectra, chemiluminescent electron transfer, and electron transfer through frozen media.
Journal ArticleDOI
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Journal ArticleDOI
The energy landscapes and motions of proteins.
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Journal ArticleDOI
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