Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
More filters
Journal ArticleDOI
Biology, one molecule at a time
TL;DR: The single-molecule approach to biology offers distinct advantages over the conventional approach of taking bulk measurements; this additional information content usually comes at the cost of the additional complexity as discussed by the authors.
Journal ArticleDOI
Statistics and Related Topics in Single-Molecule Biophysics
Hong Qian,Samuel C. Kou +1 more
TL;DR: Some important statistical developments in connection to single-molecule biophysics are reviewed, emphasizing the application of stochastic-process theory and the statistical questions arising from modeling and analyzing experimental data.
Journal ArticleDOI
Near‐Infrared Fluorescent Sensors based on Single‐Walled Carbon Nanotubes for Life Sciences Applications
Ardemis A. Boghossian,Jingqing Zhang,Paul W. Barone,Nigel F. Reuel,Jong-Ho Kim,Daniel A. Heller,Jin-Ho Ahn,Andrew J. Hilmer,Alina Y. Rwei,Jyoti R. Arkalgud,Cathy T. Zhang,Michael S. Strano +11 more
TL;DR: The design rules that are developed for detecting various analytes, ranging from stable small molecules and reactive oxygen species or reactive nitrogen species to macromolecules are described, and applications to in vivo sensor measurements using these sensors are described.
Journal ArticleDOI
Single molecule studies of enzyme mechanisms.
Journal ArticleDOI
STI-Bounded Single-Photon Avalanche Diode in a Deep-Submicrometer CMOS Technology
TL;DR: In this article, a compact CMOS Geiger-mode single-photon avalanche diode (SPAD) detector with an efficient guard ring structure for preventing edge breakdown is presented.
References
More filters
疟原虫var基因转换速率变化导致抗原变异[英]／Paul H, Robert P, Christodoulou Z, et al//Proc Natl Acad Sci U S A
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Journal ArticleDOI
Electron transfers in chemistry and biology
Rudolph A. Marcus,Norman Sutin +1 more
TL;DR: In this paper, the electron transfer reactions between ions and molecules in solution have been the subject of considerable experimental study during the past three decades, including charge transfer, photoelectric emission spectra, chemiluminescent electron transfer, and electron transfer through frozen media.
Journal ArticleDOI
Formation of glasses from liquids and biopolymers.
TL;DR: The onset of a sharp change in ddT( is the Debye-Waller factor and T is temperature) in proteins, which is controversially indentified with the glass transition in liquids, is shown to be general for glass formers and observable in computer simulations of strong and fragile ionic liquids, where it proves to be close to the experimental glass transition temperature.
Journal ArticleDOI
The energy landscapes and motions of proteins.
TL;DR: The concepts that emerge from studies of the conformational substates and the motions between them permit a quantitative discussion of one simple reaction, the binding of small ligands such as carbon monoxide to myoglobin.
Journal ArticleDOI
Fluorescence spectroscopy of single biomolecules.
TL;DR: The progress in applying single-molecule detection and single-Molecule spectroscopy at room temperature by laser-induced fluorescence with the use of fluorophores that are site-specifically attached to macromolecules is reviewed.