Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
Single-molecule spectroscopy reveals how calmodulin activates NO synthase by controlling its conformational fluctuation dynamics.
TL;DR: Using single-molecule fluorescence resonance energy transfer (FRET) spectroscopy, it is found that calmodulin binding to neuronal NO synthase reductase domain (nNOSr) both alters and restricts the distributions of NOSr conformational states and the conformational lifetimes, thereby revealing a physical means by whichCalmodulin may control electron transfer for catalysis.
Journal ArticleDOI
Single molecule photon emission statistics for non-Markovian blinking models.
Yujun Zheng,Frank L. H. Brown +1 more
TL;DR: A spectral representation is introduced to facilitate the numerical solution of the GBE equations for these more challenging cases of stochastic model systems where photon emission rates evolve in time with non-Markovian dynamics.
Journal Article
Deformability, dynamics, and remodeling of cytoskeleton of the adherent living cell.
TL;DR: Data reviewed here suggest that trapping, intermittency, and approach to kinetic arrest represent mesoscale features of collective protein-protein interactions linking underlying molecular events to integrative cellular functions such as crawling, contraction and remodeling.
Journal ArticleDOI
Unusual temperature dependence of photosynthetic electron transfer due to protein dynamics
Haiyu Wang,Su Lin,Evaldas Katilius,Christa Laser,James P. Allen,Joann Williams,Neal W. Woodbury +6 more
TL;DR: The initial electron transfer rate and protein dynamics in wild type and five mutant reaction centers from Rhodobacter sphaeroides have been studied as a function of temperature.
Journal ArticleDOI
Functional Domain Motions in Proteins on the ∼1–100 ns Timescale: Comparison of Neutron Spin-Echo Spectroscopy of Phosphoglycerate Kinase with Molecular-Dynamics Simulation
TL;DR: All-atom molecular-dynamics simulation of a protein, phosphoglycerate kinase, is reported on, from which small-angle neutron scattering (SANS) and NSE scattering properties are calculated and it is confirmed that a significant component of the NSE arises from internal dynamics.
References
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TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
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Journal ArticleDOI
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