Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
Modified single photon counting modules for optimal timing performance
TL;DR: In this paper, a modification of a standard Perkin Elmer SPCM-AQR photon detector module that remarkably improves the photon timing performance is presented, which consists of an additional timing circuit board, which is inserted in the module without modifying the original circuit board.
Journal ArticleDOI
Behavioral modeling of statistical phenomena of single-photon avalanche diodes
TL;DR: An accurate behavioral model is presented for simulating single-photon avalanche diodes (SPADs), able to emulate the static, the dynamic behavior and the main statistical effects of an SPAD, such as the turn-off probability, the dark-count and the after-pulsing phenomena.
Journal ArticleDOI
Probing the Kinetics of Single Molecule Protein Folding
TL;DR: An approach to integrate the theory, simulations, and experiments in protein-folding kinetics is proposed by measuring the mean and high-order moments of the first-passage time and its associated distribution, consistent with the strange kinetics found in folding dynamics experiments.
Journal ArticleDOI
COREX/BEST server: a web browser-based program that calculates regional stability variations within protein structures
TL;DR: The COREX/BEST Server generates a structural thermodynamic ensemble that can be used to calculate the regional variations in stability of a protein structure, and the stabilities are presented in units of energy (kcal/mol).
Journal ArticleDOI
The docking of synaptic vesicles on the presynaptic membrane induced by α-synuclein is modulated by lipid composition
Wing K. Man,Bogachan Tahirbegi,Michail D. Vrettas,Swapan Preet,Liming Ying,Michele Vendruscolo,Alfonso De Simone,Alfonso De Simone,Giuliana Fusco +8 more
TL;DR: In this article, a detailed characterisation of the conformational properties of αS bound to the inner and outer leaflets of the presynaptic plasma membrane (PM), using small unilamellar vesicles, is presented.
References
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