Journal ArticleDOI
Protein Conformational Dynamics Probed by Single-Molecule Electron Transfer
Haw Yang,Haw Yang,Haw Yang,Guobin Luo,Guobin Luo,Guobin Luo,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Pallop Karnchanaphanurach,Tai Man Louie,Tai Man Louie,Tai Man Louie,Ivan Rech,Ivan Rech,Ivan Rech,Sergio Cova,Sergio Cova,Sergio Cova,Luying Xun,Luying Xun,Luying Xun,X. Sunney Xie,X. Sunney Xie,X. Sunney Xie +23 more
TLDR
By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, the variation of flavin-tyrosine distance over time is observed, suggesting the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.Abstract:
Electron transfer is used as a probe for angstrom-scale structural changes in single protein molecules. In a flavin reductase, the fluorescence of flavin is quenched by a nearby tyrosine residue by means of photo-induced electron transfer. By probing the fluorescence lifetime of the single flavin on a photon-by-photon basis, we were able to observe the variation of flavin-tyrosine distance over time. We could then determine the potential of mean force between the flavin and the tyrosine, and a correlation analysis revealed conformational fluctuation at multiple time scales spanning from hundreds of microseconds to seconds. This phenomenon suggests the existence of multiple interconverting conformers related to the fluctuating catalytic reactivity.read more
Citations
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Journal ArticleDOI
Dynamic Disorder in Quasi-Equilibrium Enzymatic Systems
TL;DR: This paper investigates the applicability of macroscopic rate laws for fluctuating enzyme systems in which catalytic transitions are slower than ligand binding-dissociation reactions and shows that the catalytic rate has the same dependence on ligand concentrations as obtained from mass-action kinetics even in the presence of slow conformational fluctuations.
Pharmacotherapeutic receptor specificities and selectivity classes, and placebo effects: a perspective
L. Rossini,Paola Rossini +1 more
Proceedings ArticleDOI
High-detection efficiency and picosecond timing compact detector modules with red-enhanced SPADs
Andrea Giudice,Georg Simmerle,Daniele Veronese,Roberto Biasi,Angelo Gulinatti,Ivan Rech,Massimo Ghioni,Piera Maccagnani +7 more
TL;DR: In this paper, the authors present a compact photon counting and timing module that fills the gap between the high temporal resolution and the high detection efficiency systems, achieving a photon detection efficiency of 37% at 800 nm (peak of 58% at 600 nm) while maintaining a temporal resolution of about 100 ps FWHM.
Journal ArticleDOI
Single-Molecule Monitoring of the Structural Switching Dynamics of Nucleic Acids through Controlling Fluorescence Blinking
TL;DR: A method for kinetic analysis based on control of fluorescence blinking (KACB), a general approach to investigate the dynamics of biomolecules by using a single fluorophore, enabling real‐time single‐molecule measurement of the structure‐changing dynamics of nucleic acids.
Journal ArticleDOI
Ensemble-based characterization of unbound and bound states on protein energy landscape.
TL;DR: In this paper, an analysis of the unbound-to-bound changes points to conformational selection as the binding mechanism for four proteins and showed that bound and unbound spectra often significantly overlap.
References
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TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
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Rudolph A. Marcus,Norman Sutin +1 more
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Journal ArticleDOI
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Journal ArticleDOI
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Journal ArticleDOI
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